################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BanII BcgI BclI BfrI BglI BglII BlnI BmyI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI KpnI KspI MamI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 61 CGCG CG^CG AflIII 2136 ACATGT A^C[GA][CT]GT AviII 2028 TGCGCA TGC^GCA BamHI 1716 GGATCC G^GATCC BanI 1699 GGCGCC G^G[CT][GA]CC BbeI 1699 GGCGCC GGCGC^C BbrPI 2930 CACGTG CAC^GTG BbvCI 895 GCTGAGG CCTCAGC(-5/-2) BciVI 1243 GGATAC GTATCC(6/5) BlpI 1612 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1612 GCTCAGC GC^T[ACGT]AGC BsaAI 2930 CACGTG [CT]AC^GT[GA] BsmBI 1708 GAGACG CGTCTC(1/5) BspDI 2368 ATCGAT AT^CGAT BspLU11I 2136 ACATGT A^CATGT BstAPI 1476 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 61 CGCG CG^CG CelII 1612 GCTCAGC GC^T[ACGT]AGC ClaI 2368 ATCGAT AT^CGAT DraII 2050 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 517 GAAGAG CTCTTC(1/4) EcoO109I 2050 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 616 GATATC GAT^ATC FspI 2028 TGCGCA TGC^GCA HaeII 1699 GGCGCC [GA]GCGC^[CT] HindIII 2216 AAGCTT A^AGCTT HpaI 2326 GTTAAC GTT^AAC KasI 1699 GGCGCC G^GCGCC Ksp632I 517 GAAGAG CTCTTC(1/4) MfeI 1679 CAATTG C^AATTG MunI 1679 CAATTG C^AATTG MvnI 61 CGCG CG^CG NarI 1699 GGCGCC GG^CGCC NdeI 1499 CATATG CA^TATG PmaCI 2930 CACGTG CAC^GTG PmlI 2930 CACGTG CAC^GTG PpuMI 2050 GGGACCT [GA]G^G[AT]CC[CT] SfoI 1699 GGCGCC GGC^GCC SphI 961 GCATGC GCATG^C SspI 2012 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 377 GACGCC G[GA]^CG[CT]C 1699 GGCGCC BcnI 702 CCGGG CC^[GC]GG 2922 CCCGG BsaHI 377 GACGCC G[GA]^CG[CT]C 1699 GGCGCC BsaI 1008 GAGACC GGTCTC(1/5) 2402 GAGACC BseRI 2290 GAGGAG GAGGAG(10/8) 2644 GAGGAG BsmFI 2050 GGGAC GGGAC(10/14) 1213 GTCCC EcoT22I 541 ATGCAT ATGCA^T 923 ATGCAT FauI 165 CCCGC CCCGC(4/6) 823 CCCGC HapII 702 CCGG C^CGG 2923 CCGG HgaI 377 GACGC GACGC(5/10) 1933 GCGTC Hin1I 377 GACGCC G[GA]^CG[CT]C 1699 GGCGCC HincII 472 GTTGAC GT[CT]^[GA]AC 2326 GTTAAC HindII 472 GTTGAC GT[CT]^[GA]AC 2326 GTTAAC HpaII 702 CCGG C^CGG 2923 CCGG HphI 2008 GGTGA GGTGA(8/7) 1194 TCACC MaeII 2848 ACGT A^CGT 2931 ACGT MspI 702 CCGG C^CGG 2923 CCGG NciI 702 CCGGG CC^[GC]GG 2922 CCCGG NsiI 541 ATGCAT ATGCA^T 923 ATGCAT PflMI 381 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2547 CCATATCTTGG ScaI 797 AGTACT AGT^ACT 1895 AGTACT StuI 2181 AGGCCT AGG^CCT 2828 AGGCCT Van91I 381 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2547 CCATATCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BalI BbsI BbvI BfaI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsoBI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII