Restriction map for pF1KA0746
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvrII       BanII       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BmyI        
BsaBI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1286I    Bsp1407I    BspEI       
BspHI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      Cfr10I      CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       FbaI        FseI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII          61  CGCG                 CG^CG
AflIII       2136  ACATGT               A^C[GA][CT]GT
AviII        2028  TGCGCA               TGC^GCA
BamHI        1716  GGATCC               G^GATCC
BanI         1699  GGCGCC               G^G[CT][GA]CC
BbeI         1699  GGCGCC               GGCGC^C
BbrPI        2930  CACGTG               CAC^GTG
BbvCI         895  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1243  GGATAC               GTATCC(6/5)
BlpI         1612  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1612  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2930  CACGTG               [CT]AC^GT[GA]
BsmBI        1708  GAGACG               CGTCTC(1/5)
BspDI        2368  ATCGAT               AT^CGAT
BspLU11I     2136  ACATGT               A^CATGT
BstAPI       1476  GCACACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          61  CGCG                 CG^CG
CelII        1612  GCTCAGC              GC^T[ACGT]AGC
ClaI         2368  ATCGAT               AT^CGAT
DraII        2050  GGGACCT              [GA]G^G[ACGT]CC[CT]
EarI          517  GAAGAG               CTCTTC(1/4)
EcoO109I     2050  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         616  GATATC               GAT^ATC
FspI         2028  TGCGCA               TGC^GCA
HaeII        1699  GGCGCC               [GA]GCGC^[CT]
HindIII      2216  AAGCTT               A^AGCTT
HpaI         2326  GTTAAC               GTT^AAC
KasI         1699  GGCGCC               G^GCGCC
Ksp632I       517  GAAGAG               CTCTTC(1/4)
MfeI         1679  CAATTG               C^AATTG
MunI         1679  CAATTG               C^AATTG
MvnI           61  CGCG                 CG^CG
NarI         1699  GGCGCC               GG^CGCC
NdeI         1499  CATATG               CA^TATG
PmaCI        2930  CACGTG               CAC^GTG
PmlI         2930  CACGTG               CAC^GTG
PpuMI        2050  GGGACCT              [GA]G^G[AT]CC[CT]
SfoI         1699  GGCGCC               GGC^GCC
SphI          961  GCATGC               GCATG^C
SspI         2012  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          377  GACGCC               G[GA]^CG[CT]C
             1699  GGCGCC              
BcnI          702  CCGGG                CC^[GC]GG
             2922  CCCGG               
BsaHI         377  GACGCC               G[GA]^CG[CT]C
             1699  GGCGCC              
BsaI         1008  GAGACC               GGTCTC(1/5)
             2402  GAGACC              
BseRI        2290  GAGGAG               GAGGAG(10/8)
             2644  GAGGAG              
BsmFI        2050  GGGAC                GGGAC(10/14)
             1213  GTCCC               
EcoT22I       541  ATGCAT               ATGCA^T
              923  ATGCAT              
FauI          165  CCCGC                CCCGC(4/6)
              823  CCCGC               
HapII         702  CCGG                 C^CGG
             2923  CCGG                
HgaI          377  GACGC                GACGC(5/10)
             1933  GCGTC               
Hin1I         377  GACGCC               G[GA]^CG[CT]C
             1699  GGCGCC              
HincII        472  GTTGAC               GT[CT]^[GA]AC
             2326  GTTAAC              
HindII        472  GTTGAC               GT[CT]^[GA]AC
             2326  GTTAAC              
HpaII         702  CCGG                 C^CGG
             2923  CCGG                
HphI         2008  GGTGA                GGTGA(8/7)
             1194  TCACC               
MaeII        2848  ACGT                 A^CGT
             2931  ACGT                
MspI          702  CCGG                 C^CGG
             2923  CCGG                
NciI          702  CCGGG                CC^[GC]GG
             2922  CCCGG               
NsiI          541  ATGCAT               ATGCA^T
              923  ATGCAT              
PflMI         381  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2547  CCATATCTTGG         
ScaI          797  AGTACT               AGT^ACT
             1895  AGTACT              
StuI         2181  AGGCCT               AGG^CCT
             2828  AGGCCT              
Van91I        381  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2547  CCATATCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaI        AvaII       BalI        
BbsI        BbvI        BfaI        BpmI        BpuAI       
BsaJI       BsgI        BsiYI       BslI        BsmAI       
BsoBI       BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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