Restriction map for
pF1KA1082
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
BamHI BbrPI BciVI BfrI BsaAI
BsiWI BsmBI BspDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV HpaI MluI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SwaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 2825 TCCGGA T^CCGGA
AcyI 294 GGCGCC G[GA]^CG[CT]C
AflIII 5124 ACATGT A^C[GA][CT]GT
AhdI 3407 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3726 CCTGAGG CC^T[ACGT]AGG
AspEI 3407 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 307 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 125 TGCGCA TGC^GCA
AvrII 5028 CCTAGG C^CTAGG
BbeI 294 GGCGCC GGCGC^C
BglI 136 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5028 CCTAGG C^CTAGG
BsaBI 4722 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 294 GGCGCC G[GA]^CG[CT]C
BseAI 2825 TCCGGA T^CCGGA
BsiEI 1360 CGGTCG CG[GA][CT]^CG
Bsp1407I 4619 TGTACA T^GTACA
BspEI 2825 TCCGGA T^CCGGA
BspLU11I 5124 ACATGT A^CATGT
BsrGI 4619 TGTACA T^GTACA
Bsu36I 3726 CCTGAGG CC^T[ACGT]AGG
DraIII 3191 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3407 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3726 CCTGAGG CC^T[ACGT]AGG
FseI 2602 GGCCGGCC GGCCGG^CC
FspI 125 TGCGCA TGC^GCA
HaeII 294 GGCGCC [GA]GCGC^[CT]
HgaI 7 GACGC GACGC(5/10)
Hin1I 294 GGCGCC G[GA]^CG[CT]C
HincII 3383 GTCGAC GT[CT]^[GA]AC
HindII 3383 GTCGAC GT[CT]^[GA]AC
KasI 294 GGCGCC G^GCGCC
KspI 86 CCGCGG CCGC^GG
MamI 4722 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2825 TCCGGA T^CCGGA
NarI 294 GGCGCC GG^CGCC
NcoI 2156 CCATGG C^CATGG
NdeI 4404 CATATG CA^TATG
PaeR7I 131 CTCGAG C^TCGAG
PflFI 307 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3045 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4415 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 86 CCGCGG CCGC^GG
SalI 3383 GTCGAC G^TCGAC
SbfI 2694 CCTGCAGG CCTGCA^GG
SfoI 294 GGCGCC GGC^GCC
SmaI 3341 CCCGGG CCC^GGG
Sse8387I 2694 CCTGCAGG CCTGCA^GG
SspBI 4619 TGTACA T^GTACA
SspI 3316 AATATT AAT^ATT
Tth111I 307 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3045 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2949 TCTAGA T^CTAGA
XhoI 131 CTCGAG C^TCGAG
XmaCI 3341 CCCGGG C^CCGGG
XmaI 3341 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 2762 GGTACC G^GTACC
4955 GGTACC
Asp718I 2762 GGTACC G^GTACC
4955 GGTACC
BbsI 5104 GAAGAC GAAGAC(2/6)
3952 GTCTTC
BcgI 4773 CGAGGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2733 GCACAAGTGTCG
BclI 542 TGATCA T^GATCA
4926 TGATCA
BlpI 2120 GCTCAGC GC^T[ACGT]AGC
2557 GCTGAGC
Bpu1102I 2120 GCTCAGC GC^T[ACGT]AGC
2557 GCTGAGC
BpuAI 5104 GAAGAC GAAGAC(2/6)
3952 GTCTTC
BsrDI 339 CATTGC GCAATG(2/0)
4017 CATTGC
CelII 2120 GCTCAGC GC^T[ACGT]AGC
2557 GCTGAGC
EcoNI 315 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5145 CCTGACTCAGG
EcoT22I 1584 ATGCAT ATGCA^T
2709 ATGCAT
FbaI 542 TGATCA T^GATCA
4926 TGATCA
HindIII 2406 AAGCTT A^AGCTT
3814 AAGCTT
KpnI 2762 GGTACC GGTAC^C
4955 GGTACC
MaeII 787 ACGT A^CGT
2300 ACGT
MfeI 4200 CAATTG C^AATTG
4766 CAATTG
MunI 4200 CAATTG C^AATTG
4766 CAATTG
NsiI 1584 ATGCAT ATGCA^T
2709 ATGCAT
SacI 1692 GAGCTC GAGCT^C
4279 GAGCTC
SexAI 4498 ACCAGGT A^CC[AT]GGT
5066 ACCAGGT
XcmI 3023 CCATGATGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4828 CCAGGTGCTTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI