Restriction map for
pF1KA0522
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AviII BbrPI BclI BfrI
BsaAI BsaBI BsiEI BsiWI BsmI
BspHI Bst1107I BstBI BstZ17I CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MamI MfeI
MluI MseI MunI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SfiI SfuI SnaBI SpeI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 2909 GTCGAC GT^[AC][GT]AC
AhdI 2836 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 912 CCTGAGG CC^T[ACGT]AGG
Aor51HI 941 AGCGCT AGC^GCT
Asp700I 2433 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2836 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2418 CCTAGG C^CTAGG
BalI 3741 TGGCCA TGG^CCA
BciVI 2312 GTATCC GTATCC(6/5)
BglII 3041 AGATCT A^GATCT
BlnI 2418 CCTAGG C^CTAGG
BspDI 1318 ATCGAT AT^CGAT
BspLU11I 2681 ACATGT A^CATGT
BsrDI 3237 CATTGC GCAATG(2/0)
BssHII 233 GCGCGC G^CGCGC
BstAPI 99 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3015 GGTCACC G^GT[ACGT]ACC
BstPI 3015 GGTCACC G^GT[ACGT]ACC
Bsu36I 912 CCTGAGG CC^T[ACGT]AGG
ClaI 1318 ATCGAT AT^CGAT
Eam1105I 2836 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 941 AGCGCT AGC^GCT
Eco81I 912 CCTGAGG CC^T[ACGT]AGG
EcoNI 180 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3015 GGTCACC G^GT[ACGT]ACC
MluNI 3741 TGGCCA TGG^CCA
MscI 3741 TGGCCA TGG^CCA
NdeI 1292 CATATG CA^TATG
NruI 501 TCGCGA TCG^CGA
PflMI 2550 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 2909 GTCGAC G^TCGAC
SapI 3099 GCTCTTC GCTCTTC(1/4)
SbfI 984 CCTGCAGG CCTGCA^GG
SexAI 3150 ACCTGGT A^CC[AT]GGT
SphI 3801 GCATGC GCATG^C
Sse8387I 984 CCTGCAGG CCTGCA^GG
Van91I 2550 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2433 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccIII 2510 TCCGGA T^CCGGA
2624 TCCGGA
AflIII 2450 ACGTGT A^C[GA][CT]GT
2681 ACATGT
AgeI 433 ACCGGT A^CCGGT
2352 ACCGGT
BamHI 34 GGATCC G^GATCC
4219 GGATCC
BbvCI 2391 CCTCAGC CCTCAGC(-5/-2)
3932 CCTCAGC
BcgI 2911 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3231 CGAGTCCATTGC
BseAI 2510 TCCGGA T^CCGGA
2624 TCCGGA
BsmBI 3591 CGTCTC CGTCTC(1/5)
2447 GAGACG
Bsp1407I 2684 TGTACA T^GTACA
4109 TGTACA
BspEI 2510 TCCGGA T^CCGGA
2624 TCCGGA
BsrGI 2684 TGTACA T^GTACA
4109 TGTACA
FseI 1621 GGCCGGCC GGCCGG^CC
4009 GGCCGGCC
HgaI 406 GACGC GACGC(5/10)
2696 GCGTC
HincII 2752 GTTGAC GT[CT]^[GA]AC
2909 GTCGAC
HindII 2752 GTTGAC GT[CT]^[GA]AC
2909 GTCGAC
MroI 2510 TCCGGA T^CCGGA
2624 TCCGGA
PinAI 433 ACCGGT A^CCGGT
2352 ACCGGT
SacI 633 GAGCTC GAGCT^C
2100 GAGCTC
SgrAI 648 CGCCGGCG C[GA]^CCGG[CT]G
2351 CACCGGTG
SmlI 1378 CTTGAG C^T[CT][GA]AG
2095 CTTGAG
SspBI 2684 TGTACA T^GTACA
4109 TGTACA
StuI 2388 AGGCCT AGG^CCT
3680 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI