Restriction map for
pF1KB8639
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TspRI TthHB8I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AocI 200 CCTCAGG CC^T[ACGT]AGG
AspI 588 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 593 GGTCC G^G[AT]CC
BanII 644 GAGCCC G[GA]GC[CT]^C
BmyI 644 GAGCCC G[AGT]GC[ACT]^C
BsaJI 134 CCGTGG C^C[ACGT][ACGT]GG
BsgI 650 CTGCAC GTGCAG(16/14)
BsmFI 272 GTCCC GGGAC(10/14)
Bsp1286I 644 GAGCCC G[AGT]GC[ACT]^C
BspMI 526 GCAGGT ACCTGC(4/8)
BstF5I 609 GGATG GGATG(2/0)
BstYI 11 GGATCT [GA]^GATC[CT]
Bsu36I 200 CCTCAGG CC^T[ACGT]AGG
Cac8I 837 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 481 GCGC GCG^C
DsaI 134 CCGTGG C^C[GA][CT]GG
Eco81I 200 CCTCAGG CC^T[ACGT]AGG
EcoNI 109 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 794 GAATTC G^AATTC
FokI 609 GGATG GGATG(9/13)
HgaI 523 GACGC GACGC(5/10)
HhaI 481 GCGC GCG^C
HinP1I 481 GCGC G^CGC
HinfI 241 GATTC G^A[ACGT]TC
MaeII 872 ACGT A^CGT
MflI 11 GGATCT [GA]^GATC[CT]
MspA1I 227 CAGCTG C[AC]G^C[GT]G
MwoI 229 GCTGCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 588 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 592 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 227 CAGCTG CAG^CTG
SapI 457 GCTCTTC GCTCTTC(1/4)
SexAI 589 ACCAGGT A^CC[AT]GGT
TfiI 241 GATTC G^A[AT]TC
Tth111I 588 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 846 TCTAGA T^CTAGA
XhoII 11 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 474 GTAC GT^AC
900 GTAC
AlwNI 233 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
362 CAGTATCTG
BsrDI 332 GCAATG GCAATG(2/0)
830 GCAATG
BssKI 78 CCTGG ^CC[ACGT]GG
590 CCAGG
BstNI 78 CCTGG CC^[AT]GG
590 CCAGG
DraII 74 GGGGCCT [GA]G^G[ACGT]CC[CT]
592 AGGTCCT
EarI 458 CTCTTC CTCTTC(1/4)
880 GAAGAG
EcoO109I 74 GGGGCCT [GA]G^G[ACGT]CC[CT]
592 AGGTCCT
EcoRII 78 CCTGG ^CC[AT]GG
590 CCAGG
Ksp632I 458 CTCTTC CTCTTC(1/4)
880 GAAGAG
MboII 880 GAAGA GAAGA(8/7)
459 TCTTC
MvaI 78 CCTGG CC^[AT]GG
590 CCAGG
PstI 230 CTGCAG CTGCA^G
895 CTGCAG
RsaI 474 GTAC GT^AC
900 GTAC
ScrFI 78 CCTGG CC^[ACGT]GG
590 CCAGG
SfcI 230 CTGCAG C^T[GA][CT]AG
895 CTGCAG
SmlI 433 CTTGAG C^T[CT][GA]AG
930 CTTGAG
Tsp45I 304 GTCAC ^GT[GC]AC
580 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BsiYI BslI BsrI Cfr13I
DdeI DpnII Fnu4HI HaeIII HphI
ItaI MaeI MaeIII MboI MnlI
MseI NdeII NlaIII NlaIV Sau3AI
Sau96I Tru9I TseI Tsp509I