Restriction map for
pF1KB6732
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaII AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeII MaeIII MamI MfeI
MflI MluI MroI MseI MslI
MunI MvaI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 187 GGCGCC G[GA]^CG[CT]C
AhdI 149 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 240 GATCC GGATC(4/5)
AspEI 149 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 295 CTCGGG C^[CT]CG[GA]G
BalI 278 TGGCCA TGG^CCA
BanII 90 GAGCTC G[GA]GC[CT]^C
BbeI 187 GGCGCC GGCGC^C
BcgI 308 GCAGCAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 274 CTAG C^TAG
BsaHI 187 GGCGCC G[GA]^CG[CT]C
BsoBI 295 CTCGGG C^[CT]CG[GA]G
BstF5I 381 GGATG GGATG(2/0)
BstXI 203 CCAACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 120 GGCCC G^G[ACGT]CC
DpnII 240 GATC ^GATC
DraII 119 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 149 GACTCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 43 CTCTTC CTCTTC(1/4)
EcoO109I 119 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 381 GGATG GGATG(9/13)
HaeII 187 GGCGCC [GA]GCGC^[CT]
HgaI 314 GCGTC GACGC(5/10)
Hin1I 187 GGCGCC G[GA]^CG[CT]C
HinfI 149 GACTC G^A[ACGT]TC
HphI 182 TCACC GGTGA(8/7)
KasI 187 GGCGCC G^GCGCC
Ksp632I 43 CTCTTC CTCTTC(1/4)
MaeI 274 CTAG C^TAG
MboI 240 GATC ^GATC
MboII 44 TCTTC GAAGA(8/7)
MluNI 278 TGGCCA TGG^CCA
MscI 278 TGGCCA TGG^CCA
MspA1I 99 CAGCGG C[AC]G^C[GT]G
NaeI 190 GCCGGC GCC^GGC
NarI 187 GGCGCC GG^CGCC
NdeII 240 GATC ^GATC
NgoMIV 190 GCCGGC G^CCGGC
NlaIII 132 CATG CATG^
PleI 149 GACTC GAGTC(4/5)
PstI 286 CTGCAG CTGCA^G
SacI 90 GAGCTC GAGCT^C
SapI 42 GCTCTTC GCTCTTC(1/4)
Sau3AI 240 GATC ^GATC
Sau96I 120 GGCCC G^G[ACGT]CC
SfaNI 194 GCATC GCATC(5/9)
SfcI 286 CTGCAG C^T[GA][CT]AG
SfoI 187 GGCGCC GGC^GCC
SgrAI 183 CACCGGCG C[GA]^CCGG[CT]G
SspI 328 AATATT AAT^ATT
TaqI 368 TCGA T^CGA
TspRI 54 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 368 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 171 CGCG CG^CG
198 CGCG
AluI 91 AGCT AG^CT
229 AGCT
AspHI 81 GTGCTC G[AT]GC[AT]^C
90 GAGCTC
BanI 187 GGCGCC G^G[CT][GA]CC
334 GGTGCC
BsaJI 266 CCGGGG C^C[ACGT][ACGT]GG
294 CCTCGG
BsiHKAI 81 GTGCTC G[AT]GC[AT]^C
90 GAGCTC
BspMI 35 ACCTGC ACCTGC(4/8)
67 GCAGGT
BsrFI 184 ACCGGC [GA]^CCGG[CT]
190 GCCGGC
BstUI 171 CGCG CG^CG
198 CGCG
CfoI 172 GCGC GCG^C
188 GCGC
Cfr10I 184 ACCGGC [GA]^CCGG[CT]
190 GCCGGC
FauI 169 CCCGC CCCGC(4/6)
262 CCCGC
HhaI 172 GCGC GCG^C
188 GCGC
HinP1I 172 GCGC G^CGC
188 GCGC
MvnI 171 CGCG CG^CG
198 CGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI BbvI BcnI BmyI BseRI
BsiYI BslI Bsp1286I BssKI Cac8I
EaeI Fnu4HI HaeIII HapII HpaII
ItaI MnlI MspI MwoI NciI
NlaIV ScrFI TseI