Restriction map for
pF1KE0422
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseRI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HindIII HinfI HpaI KpnI
KspI MamI MfeI MflI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1132 GACGTC GACGT^C
AccIII 1089 TCCGGA T^CCGGA
AlwI 177 GATCC GGATC(4/5)
ApaI 867 GGGCCC GGGCC^C
AviII 593 TGCGCA TGC^GCA
BanI 88 GGCGCC G^G[CT][GA]CC
BbeI 88 GGCGCC GGCGC^C
BbrPI 268 CACGTG CAC^GTG
BbsI 226 GTCTTC GAAGAC(2/6)
BfaI 85 CTAG C^TAG
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaAI 268 CACGTG [CT]AC^GT[GA]
BsaWI 1089 TCCGGA [AT]^CCGG[AT]
BseAI 1089 TCCGGA T^CCGGA
BsgI 1021 CTGCAC GTGCAG(16/14)
BspEI 1089 TCCGGA T^CCGGA
BspHI 671 TCATGA T^CATGA
BspMI 368 ACCTGC ACCTGC(4/8)
BsrBI 630 GAGCGG CCGCTC(-3/-3)
BssSI 94 CACGAG CACGAG(-5/-1)
DraIII 163 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 593 TGCGCA TGC^GCA
HgaI 1151 GCGTC GACGC(5/10)
HincII 838 GTCAAC GT[CT]^[GA]AC
HindII 838 GTCAAC GT[CT]^[GA]AC
KasI 88 GGCGCC G^GCGCC
MaeI 85 CTAG C^TAG
MroI 1089 TCCGGA T^CCGGA
NaeI 25 GCCGGC GCC^GGC
NarI 88 GGCGCC GG^CGCC
NcoI 1064 CCATGG C^CATGG
NgoMIV 25 GCCGGC G^CCGGC
PaeR7I 105 CTCGAG C^TCGAG
PflMI 181 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 268 CACGTG CAC^GTG
PmlI 268 CACGTG CAC^GTG
PpuMI 98 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 705 CTGCAG CTGCA^G
RcaI 671 TCATGA T^CATGA
SacI 202 GAGCTC GAGCT^C
SfcI 705 CTGCAG C^T[GA][CT]AG
SfiI 1167 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 88 GGCGCC GGC^GCC
StuI 873 AGGCCT AGG^CCT
Tsp509I 132 AATT ^AATT
Van91I 181 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 767 CCACCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 105 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 88 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
Alw44I 265 GTGCAC G^TGCAC
655 GTGCAC
ApaLI 265 GTGCAC G^TGCAC
655 GTGCAC
AvaI 49 CTCGGG C^[CT]CG[GA]G
105 CTCGAG
BanII 202 GAGCTC G[GA]GC[CT]^C
867 GGGCCC
BpmI 697 CTCCAG CTGGAG(16/14)
529 CTGGAG
BsaHI 88 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
BsoBI 49 CTCGGG C^[CT]CG[GA]G
105 CTCGAG
BsrDI 597 CATTGC GCAATG(2/0)
302 GCAATG
BsrFI 25 GCCGGC [GA]^CCGG[CT]
937 ACCGGC
BstEII 168 GGTGACC G^GT[ACGT]ACC
462 GGTGACC
BstPI 168 GGTGACC G^GT[ACGT]ACC
462 GGTGACC
BstXI 770 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCACCCTGCTGG
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
937 ACCGGC
DdeI 146 CTAAG C^T[ACGT]AG
731 CTCAG
DraII 98 AGGTCCC [GA]G^G[ACGT]CC[CT]
866 GGGGCCC
EagI 650 CGGCCG C^GGCCG
1161 CGGCCG
EarI 259 GAAGAG CTCTTC(1/4)
289 GAAGAG
EclXI 650 CGGCCG C^GGCCG
1161 CGGCCG
Eco52I 650 CGGCCG C^GGCCG
1161 CGGCCG
EcoO109I 98 AGGTCCC [GA]G^G[ACGT]CC[CT]
866 GGGGCCC
EcoO65I 168 GGTGACC G^GT[ACGT]ACC
462 GGTGACC
EcoT14I 578 CCAAGG C^C[AT][AT]GG
1064 CCATGG
Hin1I 88 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
Ksp632I 259 GAAGAG CTCTTC(1/4)
289 GAAGAG
MspA1I 775 CAGCTG C[AC]G^C[GT]G
993 CAGCTG
NspI 213 GCATGT [GA]CATG^[CT]
238 GCATGT
PvuII 775 CAGCTG CAG^CTG
993 CAGCTG
SmlI 105 CTCGAG C^T[CT][GA]AG
1015 CTCAAG
StyI 578 CCAAGG C^C[AT][AT]GG
1064 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BalI BbvI BcgI BcnI
BglI BmyI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I