##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeIII      
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MboII       MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI           96  GCGG                 CCGC(-3/-1)
AcsI          138  AAATTT               [GA]^AATT[CT]
AflIII        115  ACATGT               A^C[GA][CT]GT
AlwNI         127  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          138  AAATTT               [GA]^AATT[CT]
AspHI          34  GTGCTC               G[AT]GC[AT]^C
BamHI         196  GGATCC               G^GATCC
BanI          169  GGTGCC               G^G[CT][GA]CC
BmyI           34  GTGCTC               G[AGT]GC[ACT]^C
BsiHKAI        34  GTGCTC               G[AT]GC[AT]^C
BsmI          219  GAATGC               GAATGC(1/-1)
Bsp1286I       34  GTGCTC               G[AGT]GC[ACT]^C
BspLU11I      115  ACATGT               A^CATGT
BssKI         132  CCTGG                ^CC[ACGT]GG
BstF5I          6  CATCC                GGATG(2/0)
BstNI         132  CCTGG                CC^[AT]GG
BstYI         196  GGATCC               [GA]^GATC[CT]
CfoI          129  GCGC                 GCG^C
DpnII         197  GATC                 ^GATC
DraI          234  TTTAAA               TTT^AAA
EcoRII        132  CCTGG                ^CC[AT]GG
FokI            6  CATCC                GGATG(9/13)
HaeII         128  AGCGCC               [GA]GCGC^[CT]
HhaI          129  GCGC                 GCG^C
HinP1I        129  GCGC                 G^CGC
MaeII         191  ACGT                 A^CGT
MboI          197  GATC                 ^GATC
MflI          196  GGATCC               [GA]^GATC[CT]
MseI          235  TTAA                 T^TAA
MvaI          132  CCTGG                CC^[AT]GG
MwoI          112  GCCACATGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          206  CATATG               CA^TATG
NdeII         197  GATC                 ^GATC
NlaIII        116  CATG                 CATG^
NspI          115  ACATGT               [GA]CATG^[CT]
Sau3AI        197  GATC                 ^GATC
ScrFI         132  CCTGG                CC^[ACGT]GG
SfaNI           5  GCATC                GCATC(5/9)
Tru9I         235  TTAA                 T^TAA
XhoII         196  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI           14  AGCT                 AG^CT
               63  AGCT                
AlwI          197  GATCC                GGATC(4/5)
              196  GGATC               
BbvI          126  GCAGC                GCAGC(8/12)
               15  GCTGC               
Fnu4HI         15  GCTGC                GC^[ACGT]GC
              126  GCAGC               
ItaI           15  GCTGC                GC^[ACGT]GC
              126  GCAGC               
MnlI          216  GAGG                 CCTC(7/6)
              229  GAGG                
NlaIV         169  GGTGCC               GG[ACGT]^[ACGT]CC
              196  GGATCC              
TseI           15  GCTGC                G^C[AT]GC
              126  GCAGC               
Tsp509I       139  AATT                 ^AATT
              253  AATT                
TspRI         108  CACTG                CA[GC]TG[ACGT][ACGT]^
              248  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################