Restriction map for
pF1KE0510
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 278 GGTACC G^GTACC
Aor51HI 186 AGCGCT AGC^GCT
ApaI 144 GGGCCC GGGCC^C
Asp718I 278 GGTACC G^GTACC
BanII 144 GGGCCC G[GA]GC[CT]^C
BglI 137 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 75 GCTAAGC GC^T[ACGT]AGC
BmyI 144 GGGCCC G[AGT]GC[ACT]^C
BpmI 268 CTCCAG CTGGAG(16/14)
Bpu1102I 75 GCTAAGC GC^T[ACGT]AGC
BsmI 357 GCATTC GAATGC(1/-1)
Bsp1286I 144 GGGCCC G[AGT]GC[ACT]^C
CelII 75 GCTAAGC GC^T[ACGT]AGC
CfoI 187 GCGC GCG^C
DpnII 681 GATC ^GATC
EaeI 46 TGGCCG [CT]^GGCC[GA]
Eco47III 186 AGCGCT AGC^GCT
HaeII 186 AGCGCT [GA]GCGC^[CT]
HhaI 187 GCGC GCG^C
HinP1I 187 GCGC G^CGC
HphI 369 GGTGA GGTGA(8/7)
KpnI 278 GGTACC GGTAC^C
MboI 681 GATC ^GATC
MseI 688 TTAA T^TAA
MspA1I 117 CCGCTG C[AC]G^C[GT]G
NdeII 681 GATC ^GATC
PaeR7I 209 CTCGAG C^TCGAG
Sau3AI 681 GATC ^GATC
SfiI 136 GGCCCATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 148 CCCGGG CCC^GGG
SmlI 209 CTCGAG C^T[CT][GA]AG
Tru9I 688 TTAA T^TAA
XhoI 209 CTCGAG C^TCGAG
XmaCI 148 CCCGGG C^CCGGG
XmaI 148 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 580 GAATTT [GA]^AATT[CT]
638 AAATTT
AfaI 279 GTAC GT^AC
417 GTAC
AlwNI 283 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
518 CAGAAGCTG
ApoI 580 GAATTT [GA]^AATT[CT]
638 AAATTT
AvaI 148 CCCGGG C^[CT]CG[GA]G
209 CTCGAG
BanI 195 GGCACC G^G[CT][GA]CC
278 GGTACC
BsaI 69 GAGACC GGTCTC(1/5)
326 GGTCTC
BsoBI 148 CCCGGG C^[CT]CG[GA]G
209 CTCGAG
BstNI 366 CCTGG CC^[AT]GG
393 CCTGG
Cac8I 96 GCAGGC GC[ACGT]^[ACGT]GC
192 GCAGGC
DraII 143 GGGGCCC [GA]G^G[ACGT]CC[CT]
144 GGGCCCC
DsaI 139 CCATGG C^C[GA][CT]GG
432 CCATGG
EcoO109I 143 GGGGCCC [GA]G^G[ACGT]CC[CT]
144 GGGCCCC
EcoRII 366 CCTGG ^CC[AT]GG
393 CCTGG
HgaI 91 GCGTC GACGC(5/10)
294 GCGTC
MvaI 366 CCTGG CC^[AT]GG
393 CCTGG
NcoI 139 CCATGG C^CATGG
432 CCATGG
PstI 190 CTGCAG CTGCA^G
289 CTGCAG
RsaI 279 GTAC GT^AC
417 GTAC
SfaNI 85 GATGC GCATC(5/9)
376 GATGC
SfcI 190 CTGCAG C^T[GA][CT]AG
289 CTGCAG
TaqI 210 TCGA T^CGA
361 TCGA
TspRI 178 CAGTG CA[GC]TG[ACGT][ACGT]^
600 CAGTG
TthHB8I 210 TCGA T^CGA
361 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BsmAI BssKI Cfr13I
DdeI EcoT14I FauI Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboII
MnlI MspI MwoI NciI NlaIII
NlaIV Sau96I ScrFI StyI TfiI
TseI Tsp509I