Restriction map for
pF1KB8961
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsmBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI DraI DraII
DraIII DrdI Eam1105I Eco81I EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI MaeI MaeIII MamI MfeI
MluI MroI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacI SalI SapI ScaI
SexAI SfaNI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 877 GAATTC [GA]^AATT[CT]
AcyI 434 GGCGCC G[GA]^CG[CT]C
AflIII 890 ACATGT A^C[GA][CT]GT
Aor51HI 833 AGCGCT AGC^GCT
ApoI 877 GAATTC [GA]^AATT[CT]
Asp700I 873 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 546 GTGCTC G[AT]GC[AT]^C
BalI 923 TGGCCA TGG^CCA
BamHI 806 GGATCC G^GATCC
BbeI 434 GGCGCC GGCGC^C
BbsI 3 GTCTTC GAAGAC(2/6)
BpmI 868 CTGGAG CTGGAG(16/14)
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaHI 434 GGCGCC G[GA]^CG[CT]C
BsiHKAI 546 GTGCTC G[AT]GC[AT]^C
BsiWI 263 CGTACG C^GTACG
BsmI 753 GCATTC GAATGC(1/-1)
BspLU11I 890 ACATGT A^CATGT
BspMI 118 GCAGGT ACCTGC(4/8)
BstYI 806 GGATCC [GA]^GATC[CT]
CpoI 387 CGGTCCG CG^G[AT]CCG
EarI 883 CTCTTC CTCTTC(1/4)
Eco47III 833 AGCGCT AGC^GCT
EcoNI 646 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 877 GAATTC G^AATTC
FseI 109 GGCCGGCC GGCCGG^CC
HgaI 858 GACGC GACGC(5/10)
Hin1I 434 GGCGCC G[GA]^CG[CT]C
HphI 899 TCACC GGTGA(8/7)
KasI 434 GGCGCC G^GCGCC
Ksp632I 883 CTCTTC CTCTTC(1/4)
MflI 806 GGATCC [GA]^GATC[CT]
MluNI 923 TGGCCA TGG^CCA
MscI 923 TGGCCA TGG^CCA
MseI 449 TTAA T^TAA
NaeI 110 GCCGGC GCC^GGC
NarI 434 GGCGCC GG^CGCC
NgoMIV 110 GCCGGC G^CCGGC
NspI 890 ACATGT [GA]CATG^[CT]
RsrII 387 CGGTCCG CG^G[AT]CCG
SfoI 434 GGCGCC GGC^GCC
SmlI 862 CTTGAG C^T[CT][GA]AG
TfiI 930 GATTC G^A[AT]TC
Tru9I 449 TTAA T^TAA
Tsp509I 878 AATT ^AATT
TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 806 GGATCC [GA]^GATC[CT]
XmnI 873 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 264 GTAC GT^AC
894 GTAC
AluI 699 AGCT AG^CT
866 AGCT
BanII 301 GAGCCC G[GA]GC[CT]^C
691 GAGCCC
BbvCI 18 CCTCAGC CCTCAGC(-5/-2)
695 CCTCAGC
BsaAI 31 TACGTG [CT]AC^GT[GA]
313 TACGTG
BsmAI 948 GAGAC GTCTC(1/5)
1022 GAGAC
BsrBI 246 GAGCGG CCGCTC(-3/-3)
464 GAGCGG
BsrFI 110 GCCGGC [GA]^CCGG[CT]
908 ACCGGC
BssHII 76 GCGCGC G^CGCGC
101 GCGCGC
BstF5I 326 GGATG GGATG(2/0)
980 GGATG
Cfr10I 110 GCCGGC [GA]^CCGG[CT]
908 ACCGGC
FokI 326 GGATG GGATG(9/13)
980 GGATG
KspI 209 CCGCGG CCGC^GG
396 CCGCGG
MboII 4 TCTTC GAAGA(8/7)
884 TCTTC
NlaIII 52 CATG CATG^
891 CATG
PleI 37 GACTC GAGTC(4/5)
562 GAGTC
PstI 116 CTGCAG CTGCA^G
607 CTGCAG
RsaI 264 GTAC GT^AC
894 GTAC
SacII 209 CCGCGG CCGC^GG
396 CCGCGG
SbfI 115 CCTGCAGG CCTGCA^GG
606 CCTGCAGG
SfcI 116 CTGCAG C^T[GA][CT]AG
607 CTGCAG
SfiI 86 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
412 GGCCCCAGCGGCC
Sse8387I 115 CCTGCAGG CCTGCA^GG
606 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AlwI AvaI AvaII
BanI BbvI BcnI BglI BmyI
BsaJI BseRI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BssSI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI SmaI TseI
XmaCI XmaI