Restriction map for
pF1KB9701
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BsgI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HinfI HphI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwNI 151 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 647 AGCGCT AGC^GCT
Asp700I 693 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 230 GGACC G^G[AT]CC
BcgI 734 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 779 AGATCT A^GATCT
BpmI 688 CTGGAG CTGGAG(16/14)
BsaWI 794 ACCGGA [AT]^CCGG[AT]
BsmFI 496 GTCCC GGGAC(10/14)
BsrFI 871 GCCGGC [GA]^CCGG[CT]
BsrI 623 CCAGT ACTGG(1/-1)
BstAPI 381 GCACGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 871 GCCGGC [GA]^CCGG[CT]
CpoI 229 CGGACCG CG^G[AT]CCG
Eco47III 647 AGCGCT AGC^GCT
EcoT14I 368 CCAAGG C^C[AT][AT]GG
HgaI 494 GCGTC GACGC(5/10)
HincII 616 GTTAAC GT[CT]^[GA]AC
HindII 616 GTTAAC GT[CT]^[GA]AC
HpaI 616 GTTAAC GTT^AAC
MaeII 401 ACGT A^CGT
MseI 617 TTAA T^TAA
MslI 834 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 871 GCCGGC GCC^GGC
NgoMIV 871 GCCGGC G^CCGGC
NotI 333 GCGGCCGC GC^GGCCGC
NspI 390 GCATGC [GA]CATG^[CT]
RsrII 229 CGGACCG CG^G[AT]CCG
SacI 9 GAGCTC GAGCT^C
SphI 390 GCATGC GCATG^C
StyI 368 CCAAGG C^C[AT][AT]GG
Tru9I 617 TTAA T^TAA
Tsp509I 853 AATT ^AATT
XmnI 693 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 600 GATCC GGATC(4/5)
845 GATCC
ApaI 114 GGGCCC GGGCC^C
372 GGGCCC
AspHI 9 GAGCTC G[AT]GC[AT]^C
930 GAGCAC
BanI 319 GGCGCC G^G[CT][GA]CC
728 GGCGCC
BbeI 319 GGCGCC GGCGC^C
728 GGCGCC
BglI 548 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
557 GCCTGAAGGGC
BseRI 951 CTCCTC GAGGAG(10/8)
61 GAGGAG
BsiEI 195 CGGCCG CG[GA][CT]^CG
334 CGGCCG
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
930 GAGCAC
BspMI 155 ACCTGC ACCTGC(4/8)
675 GCAGGT
BstYI 599 AGATCC [GA]^GATC[CT]
779 AGATCT
EagI 195 CGGCCG C^GGCCG
334 CGGCCG
EclXI 195 CGGCCG C^GGCCG
334 CGGCCG
Eco52I 195 CGGCCG C^GGCCG
334 CGGCCG
KasI 319 GGCGCC G^GCGCC
728 GGCGCC
MflI 599 AGATCC [GA]^GATC[CT]
779 AGATCT
NarI 319 GGCGCC GG^CGCC
728 GGCGCC
SfaNI 840 GATGC GCATC(5/9)
734 GCATC
SfoI 319 GGCGCC GGC^GCC
728 GGCGCC
SmaI 105 CCCGGG CCC^GGG
328 CCCGGG
XhoII 599 AGATCC [GA]^GATC[CT]
779 AGATCT
XmaCI 105 CCCGGG C^CCGGG
328 CCCGGG
XmaI 105 CCCGGG C^CCGGG
328 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanII BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI KspI MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfcI TaqI TseI TthHB8I