Restriction map for
pF1KE0516
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Cac8I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AocI 144 CCTAAGG CC^T[ACGT]AGG
Asp700I 897 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 232 CCCGGG C^[CT]CG[GA]G
AvaII 507 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanII 165 GAGCTC G[GA]GC[CT]^C
BbsI 901 GTCTTC GAAGAC(2/6)
BbvI 416 GCAGC GCAGC(8/12)
BfaI 784 CTAG C^TAG
BlpI 10 GCTCAGC GC^T[ACGT]AGC
BpmI 864 CTCCAG CTGGAG(16/14)
Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC
BpuAI 901 GTCTTC GAAGAC(2/6)
BsaAI 296 TACGTA [CT]AC^GT[GA]
BsgI 840 GTGCAG GTGCAG(16/14)
BsiYI 294 CCTACGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 294 CCTACGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 667 GTCTC GTCTC(1/5)
BsmFI 230 GTCCC GGGAC(10/14)
BsmI 805 GAATGC GAATGC(1/-1)
BsoBI 232 CCCGGG C^[CT]CG[GA]G
BspHI 701 TCATGA T^CATGA
BsrDI 809 GCAATG GCAATG(2/0)
BstNI 866 CCAGG CC^[AT]GG
Bsu36I 144 CCTAAGG CC^T[ACGT]AGG
CelII 10 GCTCAGC GC^T[ACGT]AGC
DsaI 329 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 873 GAAGAG CTCTTC(1/4)
Eco81I 144 CCTAAGG CC^T[ACGT]AGG
EcoRII 866 CCAGG ^CC[AT]GG
EcoT14I 329 CCATGG C^C[AT][AT]GG
Fnu4HI 416 GCAGC GC^[ACGT]GC
HapII 233 CCGG C^CGG
HindIII 426 AAGCTT A^AGCTT
HpaII 233 CCGG C^CGG
ItaI 416 GCAGC GC^[ACGT]GC
Ksp632I 873 GAAGAG CTCTTC(1/4)
MaeI 784 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 184 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 233 CCGG C^CGG
MvaI 866 CCAGG CC^[AT]GG
NcoI 329 CCATGG C^CATGG
PleI 88 GAGTC GAGTC(4/5)
PpuMI 506 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 701 TCATGA T^CATGA
SacI 165 GAGCTC GAGCT^C
ScaI 306 AGTACT AGT^ACT
SmaI 232 CCCGGG CCC^GGG
SmlI 511 CTTGAG C^T[CT][GA]AG
SnaBI 296 TACGTA TAC^GTA
StyI 329 CCATGG C^C[AT][AT]GG
TfiI 258 GATTC G^A[AT]TC
TseI 416 GCAGC G^C[AT]GC
XbaI 783 TCTAGA T^CTAGA
XmaCI 232 CCCGGG C^CCGGG
XmaI 232 CCCGGG C^CCGGG
XmnI 897 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 165 GAGCTC G[AT]GC[AT]^C
861 GTGCTC
BcnI 232 CCCGG CC^[GC]GG
233 CCGGG
BmyI 165 GAGCTC G[AGT]GC[ACT]^C
861 GTGCTC
BsiHKAI 165 GAGCTC G[AT]GC[AT]^C
861 GTGCTC
Bsp1286I 165 GAGCTC G[AGT]GC[ACT]^C
861 GTGCTC
BstYI 324 AGATCC [GA]^GATC[CT]
334 GGATCT
Cfr13I 150 GGGCC G^G[ACGT]CC
507 GGACC
DraI 201 TTTAAA TTT^AAA
598 TTTAAA
DraII 149 GGGGCCT [GA]G^G[ACGT]CC[CT]
506 AGGACCT
EcoO109I 149 GGGGCCT [GA]G^G[ACGT]CC[CT]
506 AGGACCT
HaeIII 3 GGCC GG^CC
151 GGCC
HinfI 88 GAGTC G^A[ACGT]TC
258 GATTC
HphI 102 GGTGA GGTGA(8/7)
345 GGTGA
MaeII 297 ACGT A^CGT
650 ACGT
MflI 324 AGATCC [GA]^GATC[CT]
334 GGATCT
MwoI 419 GCAGTACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
677 GCTTTCATAGC
NciI 232 CCCGG CC^[GC]GG
233 CCGGG
Sau96I 150 GGGCC G^G[ACGT]CC
507 GGACC
XhoII 324 AGATCC [GA]^GATC[CT]
334 GGATCT
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BsaJI BsrI BssKI BstF5I DdeI
DpnII FokI MaeIII MboI MboII
MnlI MseI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI Tru9I
Tsp45I Tsp509I TspRI