Restriction map for
pF1KB6495
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanII BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I MaeIII MamI MfeI
MluI MroI MunI MvaI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 274 AAATTT [GA]^AATT[CT]
AluI 232 AGCT AG^CT
ApoI 274 AAATTT [GA]^AATT[CT]
AspHI 394 GAGCAC G[AT]GC[AT]^C
AvaI 141 CCCGAG C^[CT]CG[GA]G
BalI 828 TGGCCA TGG^CCA
BciVI 237 GGATAC GTATCC(6/5)
BmyI 394 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 394 GAGCAC G[AT]GC[AT]^C
BsmAI 31 GAGAC GTCTC(1/5)
BsmBI 31 GAGACG CGTCTC(1/5)
BsmFI 67 GTCCC GGGAC(10/14)
BsoBI 141 CCCGAG C^[CT]CG[GA]G
Bsp1286I 394 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 295 TGTACA T^GTACA
BsrBI 144 GAGCGG CCGCTC(-3/-3)
BsrFI 84 GCCGGC [GA]^CCGG[CT]
BsrGI 295 TGTACA T^GTACA
BstF5I 405 GGATG GGATG(2/0)
BstXI 816 CCAGCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
EagI 86 CGGCCG C^GGCCG
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
FokI 405 GGATG GGATG(9/13)
HgaI 202 GACGC GACGC(5/10)
HincII 289 GTTGAC GT[CT]^[GA]AC
HindII 289 GTTGAC GT[CT]^[GA]AC
MaeII 822 ACGT A^CGT
MluNI 828 TGGCCA TGG^CCA
MscI 828 TGGCCA TGG^CCA
MslI 435 CATGGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 84 GCCGGC GCC^GGC
NgoMIV 84 GCCGGC G^CCGGC
PleI 79 GAGTC GAGTC(4/5)
ScaI 515 AGTACT AGT^ACT
SfaNI 358 GATGC GCATC(5/9)
SspBI 295 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 462 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
639 GAAAAAGTTC
AvaII 378 GGTCC G^G[AT]CC
450 GGACC
BamHI 309 GGATCC G^GATCC
561 GGATCC
BcnI 89 CCGGG CC^[GC]GG
453 CCCGG
BfaI 242 CTAG C^TAG
382 CTAG
BpmI 119 CTGGAG CTGGAG(16/14)
634 CTGGAG
BseRI 16 GAGGAG GAGGAG(10/8)
196 GAGGAG
BsiEI 86 CGGCCG CG[GA][CT]^CG
128 CGACCG
BsrI 319 ACTGG ACTGG(1/-1)
365 CCAGT
BssKI 89 CCGGG ^CC[ACGT]GG
453 CCCGG
BstYI 309 GGATCC [GA]^GATC[CT]
561 GGATCC
DdeI 229 CTGAG C^T[ACGT]AG
497 CTGAG
DraI 255 TTTAAA TTT^AAA
604 TTTAAA
DraII 377 AGGTCCT [GA]G^G[ACGT]CC[CT]
449 AGGACCC
EcoO109I 377 AGGTCCT [GA]G^G[ACGT]CC[CT]
449 AGGACCC
EcoT14I 26 CCATGG C^C[AT][AT]GG
434 CCATGG
HphI 456 GGTGA GGTGA(8/7)
599 TCACC
KspI 107 CCGCGG CCGC^GG
131 CCGCGG
MaeI 242 CTAG C^TAG
382 CTAG
MflI 309 GGATCC [GA]^GATC[CT]
561 GGATCC
MseI 256 TTAA T^TAA
605 TTAA
NciI 89 CCGGG CC^[GC]GG
453 CCCGG
NcoI 26 CCATGG C^CATGG
434 CCATGG
PpuMI 377 AGGTCCT [GA]G^G[AT]CC[CT]
449 AGGACCC
SacII 107 CCGCGG CCGC^GG
131 CCGCGG
ScrFI 89 CCGGG CC^[ACGT]GG
453 CCCGG
StyI 26 CCATGG C^C[AT][AT]GG
434 CCATGG
TfiI 150 GAATC G^A[AT]TC
483 GAATC
Tru9I 256 TTAA T^TAA
605 TTAA
Tsp509I 275 AATT ^AATT
551 AATT
XcmI 648 CCATACTGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
816 CCAGCGACGTGGTGG
XhoII 309 GGATCC [GA]^GATC[CT]
561 GGATCC
XmnI 462 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
639 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcyI AfaI AlwI
BanI BbeI BbvI BsaHI BsaJI
BsiYI BslI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EaeI FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MboI MboII MnlI MspA1I
MspI MvnI MwoI NarI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
SfoI TseI TspRI