Restriction map for
pF1KB7748
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI DraI DraIII DsaI EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AflIII 761 ACACGT A^C[GA][CT]GT
AgeI 610 ACCGGT A^CCGGT
AhdI 1138 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 631 GTGCAC G^TGCAC
Aor51HI 1417 AGCGCT AGC^GCT
ApaLI 631 GTGCAC G^TGCAC
AspEI 1138 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI 679 CTAG C^TAG
BglII 591 AGATCT A^GATCT
BsaAI 898 TACGTG [CT]AC^GT[GA]
BsaWI 610 ACCGGT [AT]^CCGG[AT]
BsmAI 336 GTCTC GTCTC(1/5)
BspMI 854 ACCTGC ACCTGC(4/8)
CpoI 911 CGGACCG CG^G[AT]CCG
DrdI 559 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1138 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1417 AGCGCT AGC^GCT
MaeI 679 CTAG C^TAG
MboII 668 TCTTC GAAGA(8/7)
PflMI 1051 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 610 ACCGGT A^CCGGT
PpuMI 771 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 922 CAGCTG CAG^CTG
RsrII 911 CGGACCG CG^G[AT]CCG
SgrAI 153 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 425 CCCGGG CCC^GGG
TfiI 888 GATTC G^A[AT]TC
Van91I 1051 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 678 TCTAGA T^CTAGA
XmaCI 425 CCCGGG C^CCGGG
XmaI 425 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 813 GTAC GT^AC
897 GTAC
AlwNI 42 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
328 CAGCGGCTG
AspHI 631 GTGCAC G[AT]GC[AT]^C
784 GTGCTC
BcgI 693 CGAGGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
193 GCAGGGGCCTCG
BpmI 507 CTCCAG CTGGAG(16/14)
871 CTGGAG
BseRI 66 CTCCTC GAGGAG(10/8)
1092 GAGGAG
BsgI 148 CTGCAC GTGCAG(16/14)
1201 CTGCAC
BsiHKAI 631 GTGCAC G[AT]GC[AT]^C
784 GTGCTC
BsrBI 340 CCGCTC CCGCTC(-3/-3)
1174 GAGCGG
BssHII 837 GCGCGC G^CGCGC
1448 GCGCGC
BstF5I 983 GGATG GGATG(2/0)
1074 GGATG
BstXI 284 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
480 CCACCACAGTGG
FokI 983 GGATG GGATG(9/13)
1074 GGATG
FseI 72 GGCCGGCC GGCCGG^CC
657 GGCCGGCC
HgaI 459 GACGC GACGC(5/10)
949 GACGC
MaeIII 901 GTGAC ^GT[ACGT]AC
1231 GTCAC
MslI 285 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
481 CACCACAGTG
RsaI 813 GTAC GT^AC
897 GTAC
StuI 642 AGGCCT AGG^CCT
867 AGGCCT
TaqI 279 TCGA T^CGA
724 TCGA
Tsp45I 901 GTGAC ^GT[GC]AC
1231 GTCAC
TthHB8I 279 TCGA T^CGA
724 TCGA
XcmI 284 CCACCCCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1443 CCAAGGCGCGCTTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI ApoI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MboI MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI Tru9I TseI
Tsp509I TspRI XhoII