Restriction map for
pF1KB6353
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MboI
MfeI MflI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
Sau3AI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 482 TCCGGA T^CCGGA
AocI 376 CCTAAGG CC^T[ACGT]AGG
AscI 299 GGCGCGCC GG^CGCGCC
BbsI 292 GAAGAC GAAGAC(2/6)
BciVI 390 GTATCC GTATCC(6/5)
BlpI 63 GCTGAGC GC^T[ACGT]AGC
BpmI 20 CTGGAG CTGGAG(16/14)
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BpuAI 292 GAAGAC GAAGAC(2/6)
BsaWI 482 TCCGGA [AT]^CCGG[AT]
BseAI 482 TCCGGA T^CCGGA
BseRI 190 GAGGAG GAGGAG(10/8)
BspEI 482 TCCGGA T^CCGGA
BspMI 601 ACCTGC ACCTGC(4/8)
BsrBI 151 GAGCGG CCGCTC(-3/-3)
BssHII 300 GCGCGC G^CGCGC
BssSI 790 CTCGTG CACGAG(-5/-1)
Bsu36I 376 CCTAAGG CC^T[ACGT]AGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
DraII 400 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 305 CCGCGG C^C[GA][CT]GG
Eco81I 376 CCTAAGG CC^T[ACGT]AGG
EcoO109I 400 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 723 CCTTGG C^C[AT][AT]GG
FauI 153 GCGGG CCCGC(4/6)
HgaI 256 GACGC GACGC(5/10)
KspI 305 CCGCGG CCGC^GG
MroI 482 TCCGGA T^CCGGA
NlaIII 33 CATG CATG^
PflMI 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 400 AGGACCC [GA]G^G[AT]CC[CT]
SacI 66 GAGCTC GAGCT^C
SacII 305 CCGCGG CCGC^GG
SapI 717 GCTCTTC GCTCTTC(1/4)
ScaI 263 AGTACT AGT^ACT
StuI 522 AGGCCT AGG^CCT
StyI 723 CCTTGG C^C[AT][AT]GG
Van91I 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 301 CGCG CG^CG
306 CGCG
AfaI 225 GTAC GT^AC
264 GTAC
AlwNI 406 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
553 CAGACCCTG
AvaII 401 GGACC G^G[AT]CC
545 GGACC
BanII 66 GAGCTC G[GA]GC[CT]^C
630 GAGCCC
BcnI 228 CCGGG CC^[GC]GG
731 CCGGG
BsmAI 173 GAGAC GTCTC(1/5)
539 GAGAC
BsmFI 89 GGGAC GGGAC(10/14)
145 GGGAC
BstUI 301 CGCG CG^CG
306 CGCG
Cac8I 300 GCGCGC GC[ACGT]^[ACGT]GC
713 GCCTGC
EarI 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
Ksp632I 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
MaeII 102 ACGT A^CGT
215 ACGT
MspA1I 305 CCGCGG C[AC]G^C[GT]G
361 CAGCGG
MvnI 301 CGCG CG^CG
306 CGCG
NciI 228 CCGGG CC^[GC]GG
731 CCGGG
PleI 788 GACTC GAGTC(4/5)
692 GAGTC
PstI 321 CTGCAG CTGCA^G
412 CTGCAG
RsaI 225 GTAC GT^AC
264 GTAC
SfaNI 564 GATGC GCATC(5/9)
681 GATGC
SfcI 321 CTGCAG C^T[GA][CT]AG
412 CTGCAG
TaqI 206 TCGA T^CGA
582 TCGA
TthHB8I 206 TCGA T^CGA
582 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI Asp700I AspHI BbvI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MspI MvaI MwoI NlaIV
Sau96I ScrFI TfiI TseI Tsp45I
TspRI XmnI