Restriction map for
pF1KE0996
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BlnI
BsaAI BseAI BsiEI BsiWI BsoBI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 1384 GGCGTC G[GA]^CG[CT]C
AhdI 858 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 100 GTGCAC G^TGCAC
ApaLI 100 GTGCAC G^TGCAC
AspEI 858 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1069 GAGCTC G[GA]GC[CT]^C
BciVI 1171 GGATAC GTATCC(6/5)
BclI 1292 TGATCA T^GATCA
BglII 587 AGATCT A^GATCT
BlpI 871 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 871 GCTGAGC GC^T[ACGT]AGC
BsaBI 1172 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1384 GGCGTC G[GA]^CG[CT]C
BsaI 1559 GAGACC GGTCTC(1/5)
BsmBI 1843 CGTCTC CGTCTC(1/5)
Bsp1407I 1889 TGTACA T^GTACA
BspDI 442 ATCGAT AT^CGAT
BspHI 1343 TCATGA T^CATGA
BsrDI 1827 CATTGC GCAATG(2/0)
BsrGI 1889 TGTACA T^GTACA
BssSI 761 CACGAG CACGAG(-5/-1)
BstYI 587 AGATCT [GA]^GATC[CT]
CelII 871 GCTGAGC GC^T[ACGT]AGC
ClaI 442 ATCGAT AT^CGAT
Eam1105I 858 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1321 GAATTC G^AATTC
FbaI 1292 TGATCA T^GATCA
Hin1I 1384 GGCGTC G[GA]^CG[CT]C
HindIII 519 AAGCTT A^AGCTT
MamI 1172 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 587 AGATCT [GA]^GATC[CT]
NdeI 1790 CATATG CA^TATG
NspI 674 ACATGC [GA]CATG^[CT]
PflMI 495 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1343 TCATGA T^CATGA
SacI 1069 GAGCTC GAGCT^C
SmlI 980 CTCAAG C^T[CT][GA]AG
SspBI 1889 TGTACA T^GTACA
Tsp45I 805 GTCAC ^GT[GC]AC
Van91I 495 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1953 TCTAGA T^CTAGA
XhoII 587 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 947 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1486 CAGCAGCTG
AocI 574 CCTGAGG CC^T[ACGT]AGG
1455 CCTGAGG
BalI 1280 TGGCCA TGG^CCA
1430 TGGCCA
BseRI 218 CTCCTC GAGGAG(10/8)
577 GAGGAG
BsgI 1389 CTGCAC GTGCAG(16/14)
1372 GTGCAG
BsmI 684 GAATGC GAATGC(1/-1)
1146 GAATGC
BstXI 257 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1678 CCAGTCCGGTGG
Bsu36I 574 CCTGAGG CC^T[ACGT]AGG
1455 CCTGAGG
DsaI 748 CCATGG C^C[GA][CT]GG
1523 CCATGG
Eco81I 574 CCTGAGG CC^T[ACGT]AGG
1455 CCTGAGG
HaeII 80 AGCGCC [GA]GCGC^[CT]
1400 AGCGCC
HgaI 1385 GCGTC GACGC(5/10)
1920 GCGTC
MaeIII 797 GTAAC ^GT[ACGT]AC
805 GTCAC
MluNI 1280 TGGCCA TGG^CCA
1430 TGGCCA
MscI 1280 TGGCCA TGG^CCA
1430 TGGCCA
MspA1I 700 CAGCTG C[AC]G^C[GT]G
1489 CAGCTG
NcoI 748 CCATGG C^CATGG
1523 CCATGG
PpuMI 974 GGGACCC [GA]G^G[AT]CC[CT]
1199 AGGACCT
PstI 1097 CTGCAG CTGCA^G
1160 CTGCAG
PvuII 700 CAGCTG CAG^CTG
1489 CAGCTG
SspI 719 AATATT AAT^ATT
1948 AATATT
TaqI 443 TCGA T^CGA
1572 TCGA
TthHB8I 443 TCGA T^CGA
1572 TCGA
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI
XcmI