Restriction map for
pF1KA1926
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbeI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaI BseRI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MflI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 289 GACGTC GACGT^C
AccI 1081 GTCTAC GT^[AC][GT]AC
AccIII 859 TCCGGA T^CCGGA
Alw44I 109 GTGCAC G^TGCAC
AlwI 1177 GATCC GGATC(4/5)
ApaLI 109 GTGCAC G^TGCAC
AspI 238 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 899 TGGCCA TGG^CCA
BbrPI 112 CACGTG CAC^GTG
BbsI 292 GTCTTC GAAGAC(2/6)
BlpI 346 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 346 GCTGAGC GC^T[ACGT]AGC
BpuAI 292 GTCTTC GAAGAC(2/6)
BsaAI 112 CACGTG [CT]AC^GT[GA]
BseAI 859 TCCGGA T^CCGGA
BsiEI 893 CGGCCG CG[GA][CT]^CG
BsmI 322 GCATTC GAATGC(1/-1)
BspEI 859 TCCGGA T^CCGGA
BsrFI 671 ACCGGC [GA]^CCGG[CT]
BsrI 620 ACTGG ACTGG(1/-1)
CelII 346 GCTGAGC GC^T[ACGT]AGC
Cfr10I 671 ACCGGC [GA]^CCGG[CT]
DpnII 1177 GATC ^GATC
EagI 893 CGGCCG C^GGCCG
EarI 1072 CTCTTC CTCTTC(1/4)
EclXI 893 CGGCCG C^GGCCG
Eco52I 893 CGGCCG C^GGCCG
EcoT14I 902 CCAAGG C^C[AT][AT]GG
HinfI 127 GAGTC G^A[ACGT]TC
Ksp632I 1072 CTCTTC CTCTTC(1/4)
MboI 1177 GATC ^GATC
MluNI 899 TGGCCA TGG^CCA
MroI 859 TCCGGA T^CCGGA
MscI 899 TGGCCA TGG^CCA
NdeII 1177 GATC ^GATC
NlaIII 462 CATG CATG^
PflFI 238 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 127 GAGTC GAGTC(4/5)
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
SapI 1071 GCTCTTC GCTCTTC(1/4)
Sau3AI 1177 GATC ^GATC
SmaI 677 CCCGGG CCC^GGG
SmlI 250 CTCAAG C^T[CT][GA]AG
StuI 1027 AGGCCT AGG^CCT
StyI 902 CCAAGG C^C[AT][AT]GG
Tth111I 238 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 677 CCCGGG C^CCGGG
XmaI 677 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 145 GTAC GT^AC
850 GTAC
AlwNI 71 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
152 CAGCGCCTG
AspHI 109 GTGCAC G[AT]GC[AT]^C
1103 GAGCAC
AvaI 11 CTCGGG C^[CT]CG[GA]G
677 CCCGGG
AviII 746 TGCGCA TGC^GCA
1037 TGCGCA
BanI 184 GGCACC G^G[CT][GA]CC
1116 GGCACC
BanII 132 GGGCTC G[GA]GC[CT]^C
682 GAGCCC
BfaI 304 CTAG C^TAG
943 CTAG
BpmI 1097 CTCCAG CTGGAG(16/14)
118 CTGGAG
BsaWI 859 TCCGGA [AT]^CCGG[AT]
1107 ACCGGA
BsiHKAI 109 GTGCAC G[AT]GC[AT]^C
1103 GAGCAC
BsmFI 703 GGGAC GGGAC(10/14)
26 GTCCC
BsoBI 11 CTCGGG C^[CT]CG[GA]G
677 CCCGGG
BspMI 809 ACCTGC ACCTGC(4/8)
162 GCAGGT
BstF5I 570 CATCC GGATG(2/0)
372 GGATG
DdeI 347 CTGAG C^T[ACGT]AG
651 CTCAG
DraII 306 AGGCCCC [GA]G^G[ACGT]CC[CT]
740 GGGGCCT
EcoO109I 306 AGGCCCC [GA]G^G[ACGT]CC[CT]
740 GGGGCCT
FokI 570 CATCC GGATG(9/13)
372 GGATG
FspI 746 TGCGCA TGC^GCA
1037 TGCGCA
HaeII 153 AGCGCC [GA]GCGC^[CT]
416 GGCGCT
KspI 44 CCGCGG CCGC^GG
956 CCGCGG
MaeI 304 CTAG C^TAG
943 CTAG
MslI 175 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
502 CACCCCCGTG
RsaI 145 GTAC GT^AC
850 GTAC
SacII 44 CCGCGG CCGC^GG
956 CCGCGG
TspRI 653 CAGTG CA[GC]TG[ACGT][ACGT]^
978 CAGTG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BcgI BcnI BmyI BsaHI
BsaJI BsgI BsiYI BslI Bsp1286I
BssHII BssKI BstNI BstUI BstXI
Cac8I CfoI Cfr13I DsaI EaeI
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PstI PvuII
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I