Restriction map for
pF1KB8892
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
MaeIII MamI MfeI MflI MluI
MroI MslI MunI NarI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI SacI SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 223 GACGCC G[GA]^CG[CT]C
AlwI 472 GATCC GGATC(4/5)
ApaI 573 GGGCCC GGGCC^C
Asp700I 553 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 54 TGCGCA TGC^GCA
BalI 449 TGGCCA TGG^CCA
BfaI 412 CTAG C^TAG
BlpI 138 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC
BsaHI 223 GACGCC G[GA]^CG[CT]C
BsmAI 312 GAGAC GTCTC(1/5)
BsmBI 312 GAGACG CGTCTC(1/5)
BsmI 337 GCATTC GAATGC(1/-1)
BsrBI 307 GAGCGG CCGCTC(-3/-3)
BsrI 577 CCAGT ACTGG(1/-1)
BssSI 7 CTCGTG CACGAG(-5/-1)
BstAPI 57 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 138 GCTGAGC GC^T[ACGT]AGC
CpoI 87 CGGTCCG CG^G[AT]CCG
DsaI 188 CCGCGG C^C[GA][CT]GG
EcoT14I 452 CCAAGG C^C[AT][AT]GG
FspI 54 TGCGCA TGC^GCA
Hin1I 223 GACGCC G[GA]^CG[CT]C
HinfI 402 GACTC G^A[ACGT]TC
KspI 188 CCGCGG CCGC^GG
MaeI 412 CTAG C^TAG
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
NlaIII 63 CATG CATG^
NotI 190 GCGGCCGC GC^GGCCGC
PleI 402 GACTC GAGTC(4/5)
PpuMI 294 AGGACCC [GA]G^G[AT]CC[CT]
RsrII 87 CGGTCCG CG^G[AT]CCG
SacII 188 CCGCGG CCGC^GG
SfiI 268 GGCCGTCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 244 CCCGGG CCC^GGG
SmlI 496 CTCAAG C^T[CT][GA]AG
StyI 452 CCAAGG C^C[AT][AT]GG
TaqI 581 TCGA T^CGA
TspRI 318 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 581 TCGA T^CGA
XmaCI 244 CCCGGG C^CCGGG
XmaI 244 CCCGGG C^CCGGG
XmnI 553 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 244 CCCGGG C^[CT]CG[GA]G
475 CCCGAG
BglI 269 GCCGTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
481 GCCTTCAAGGC
BpmI 253 CTGGAG CTGGAG(16/14)
631 CTGGAG
BsoBI 244 CCCGGG C^[CT]CG[GA]G
475 CCCGAG
DpnII 396 GATC ^GATC
472 GATC
DraII 115 AGGCCCT [GA]G^G[ACGT]CC[CT]
294 AGGACCC
EarI 82 CTCTTC CTCTTC(1/4)
585 GAAGAG
EcoO109I 115 AGGCCCT [GA]G^G[ACGT]CC[CT]
294 AGGACCC
HaeII 258 GGCGCT [GA]GCGC^[CT]
627 GGCGCT
HphI 30 TCACC GGTGA(8/7)
39 TCACC
Ksp632I 82 CTCTTC CTCTTC(1/4)
585 GAAGAG
MaeII 368 ACGT A^CGT
443 ACGT
MboI 396 GATC ^GATC
472 GATC
MboII 585 GAAGA GAAGA(8/7)
83 TCTTC
MspA1I 188 CCGCGG C[AC]G^C[GT]G
194 CCGCTG
NaeI 278 GCCGGC GCC^GGC
372 GCCGGC
NdeII 396 GATC ^GATC
472 GATC
NgoMIV 278 GCCGGC G^CCGGC
372 GCCGGC
NlaIV 295 GGACCC GG[ACGT]^[ACGT]CC
573 GGGCCC
PstI 197 CTGCAG CTGCA^G
541 CTGCAG
Sau3AI 396 GATC ^GATC
472 GATC
SfaNI 457 GATGC GCATC(5/9)
359 GCATC
SfcI 197 CTGCAG C^T[GA][CT]AG
541 CTGCAG
StuI 229 AGGCCT AGG^CCT
479 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiEI
BsiYI BslI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MnlI MseI
MspI MvaI MvnI MwoI NciI
Sau96I ScrFI Tru9I TseI