Restriction map for
pF1KB6499
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeIII
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I TseI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 262 GTCTAC GT^[AC][GT]AC
AcyI 107 GGCGCC G[GA]^CG[CT]C
AspHI 223 GAGCAC G[AT]GC[AT]^C
AvaII 63 GGTCC G^G[AT]CC
BanI 107 GGCGCC G^G[CT][GA]CC
BbeI 107 GGCGCC GGCGC^C
BlpI 46 GCTAAGC GC^T[ACGT]AGC
BmyI 223 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC
BsaHI 107 GGCGCC G[GA]^CG[CT]C
BsaI 145 GGTCTC GGTCTC(1/5)
BsaJI 81 CCAGGG C^C[ACGT][ACGT]GG
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C
BsrI 30 ACTGG ACTGG(1/-1)
BssKI 81 CCAGG ^CC[ACGT]GG
BstNI 81 CCAGG CC^[AT]GG
CelII 46 GCTAAGC GC^T[ACGT]AGC
Cfr13I 63 GGTCC G^G[ACGT]CC
DdeI 47 CTAAG C^T[ACGT]AG
DraII 62 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 273 GAAGAG CTCTTC(1/4)
EcoO109I 62 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 81 CCAGG ^CC[AT]GG
HapII 105 CCGG C^CGG
Hin1I 107 GGCGCC G[GA]^CG[CT]C
HpaII 105 CCGG C^CGG
HphI 54 TCACC GGTGA(8/7)
KasI 107 GGCGCC G^GCGCC
Ksp632I 273 GAAGAG CTCTTC(1/4)
MaeII 194 ACGT A^CGT
MboII 273 GAAGA GAAGA(8/7)
MspI 105 CCGG C^CGG
MvaI 81 CCAGG CC^[AT]GG
NarI 107 GGCGCC GG^CGCC
NlaIV 107 GGCGCC GG[ACGT]^[ACGT]CC
PleI 162 GACTC GAGTC(4/5)
PpuMI 62 AGGTCCT [GA]G^G[AT]CC[CT]
Sau96I 63 GGTCC G^G[ACGT]CC
ScrFI 81 CCAGG CC^[ACGT]GG
SfaNI 205 GATGC GCATC(5/9)
SfoI 107 GGCGCC GGC^GCC
TfiI 198 GATTC G^A[AT]TC
TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 10 enzymes) #
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BsmFI 27 GGGAC GGGAC(10/14)
282 GGGAC
Cac8I 96 GCCTGC GC[ACGT]^[ACGT]GC
225 GCACGC
FauI 122 GCGGG CCCGC(4/6)
167 GCGGG
Fnu4HI 11 GCGGC GC^[ACGT]GC
305 GCGGC
HaeII 43 GGCGCT [GA]GCGC^[CT]
107 GGCGCC
HgaI 284 GACGC GACGC(5/10)
51 GCGTC
HinfI 162 GACTC G^A[ACGT]TC
198 GATTC
ItaI 11 GCGGC GC^[ACGT]GC
305 GCGGC
MnlI 41 GAGG CCTC(7/6)
157 GAGG
MwoI 11 GCGGCAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
299 GCTTCGGCGGC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AccII AciI BsmAI BstF5I BstUI
CfoI FokI HhaI HinP1I MaeIII
MvnI Tsp45I