Restriction map for
pF1KB6795
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI AluI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr13I ClaI CpoI DraI
DraII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HinfI HpaI KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SapI Sau96I SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 6 GTCGAC GT^[AC][GT]AC
AflIII 248 ACATGT A^C[GA][CT]GT
AlwNI 104 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 172 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 46 CTCGAG C^[CT]CG[GA]G
BalI 206 TGGCCA TGG^CCA
BcnI 58 CCGGG CC^[GC]GG
BfaI 217 CTAG C^TAG
BsaAI 267 CACGTA [CT]AC^GT[GA]
BseRI 226 CTCCTC GAGGAG(10/8)
BsgI 311 GTGCAG GTGCAG(16/14)
BsoBI 46 CTCGAG C^[CT]CG[GA]G
BspLU11I 248 ACATGT A^CATGT
BspMI 187 GCAGGT ACCTGC(4/8)
BsrFI 254 ACCGGC [GA]^CCGG[CT]
BstNI 115 CCAGG CC^[AT]GG
CfoI 243 GCGC GCG^C
Cfr10I 254 ACCGGC [GA]^CCGG[CT]
DraIII 27 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 172 GACATTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 229 CTCTTC CTCTTC(1/4)
EcoNI 109 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 115 CCAGG ^CC[AT]GG
EcoT14I 287 CCAAGG C^C[AT][AT]GG
EcoT22I 85 ATGCAT ATGCA^T
HhaI 243 GCGC GCG^C
HinP1I 243 GCGC G^CGC
HincII 6 GTCGAC GT[CT]^[GA]AC
HindII 6 GTCGAC GT[CT]^[GA]AC
HphI 355 GGTGA GGTGA(8/7)
Ksp632I 229 CTCTTC CTCTTC(1/4)
MaeI 217 CTAG C^TAG
MaeII 268 ACGT A^CGT
MboII 230 TCTTC GAAGA(8/7)
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MvaI 115 CCAGG CC^[AT]GG
NciI 58 CCGGG CC^[GC]GG
NsiI 85 ATGCAT ATGCA^T
NspI 248 ACATGT [GA]CATG^[CT]
PaeR7I 46 CTCGAG C^TCGAG
PflFI 172 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 325 CTGCAG CTGCA^G
SalI 6 GTCGAC G^TCGAC
SbfI 324 CCTGCAGG CCTGCA^GG
ScaI 374 AGTACT AGT^ACT
SfcI 325 CTGCAG C^T[GA][CT]AG
SmlI 46 CTCGAG C^T[CT][GA]AG
Sse8387I 324 CCTGCAGG CCTGCA^GG
StyI 287 CCAAGG C^C[AT][AT]GG
Tth111I 172 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 46 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 55 GATCC GGATC(4/5)
112 GATCC
BmyI 264 GGGCAC G[AGT]GC[ACT]^C
291 GGGCAC
BsaI 148 GAGACC GGTCTC(1/5)
332 GAGACC
Bsp1286I 264 GGGCAC G[AGT]GC[ACT]^C
291 GGGCAC
BsrDI 99 CATTGC GCAATG(2/0)
183 CATTGC
BssKI 58 CCGGG ^CC[ACGT]GG
115 CCAGG
BstXI 161 CCACCATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
345 CCAAGATGCTGG
DdeI 40 CTCAG C^T[ACGT]AG
305 CTGAG
DpnII 55 GATC ^GATC
112 GATC
EaeI 130 TGGCCG [CT]^GGCC[GA]
206 TGGCCA
MboI 55 GATC ^GATC
112 GATC
NdeII 55 GATC ^GATC
112 GATC
NlaIII 237 CATG CATG^
249 CATG
Sau3AI 55 GATC ^GATC
112 GATC
ScrFI 58 CCGGG CC^[ACGT]GG
115 CCAGG
SfaNI 316 GATGC GCATC(5/9)
349 GATGC
TaqI 7 TCGA T^CGA
47 TCGA
TthHB8I 7 TCGA T^CGA
47 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI BbvI BsaJI BsiYI BslI
BsmAI BstF5I Fnu4HI FokI HaeIII
HapII HpaII ItaI MnlI MslI
MspI MwoI RsaI TseI