Restriction map for
pF1KB6401
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII HphI KasI KpnI
KspI MaeII MamI MluI MroI
MslI MspA1I MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AvaII 753 GGACC G^G[AT]CC
BalI 423 TGGCCA TGG^CCA
BbsI 684 GTCTTC GAAGAC(2/6)
BbvI 796 GCAGC GCAGC(8/12)
BcgI 390 CGAAAAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 733 AGATCT A^GATCT
BpuAI 684 GTCTTC GAAGAC(2/6)
BsgI 302 GTGCAG GTGCAG(16/14)
BsiYI 604 CCTCCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 604 CCTCCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 887 GTCTC GTCTC(1/5)
BsrDI 701 GCAATG GCAATG(2/0)
BssKI 438 CCTGG ^CC[ACGT]GG
BstF5I 38 GGATG GGATG(2/0)
BstNI 438 CCTGG CC^[AT]GG
BstYI 733 AGATCT [GA]^GATC[CT]
Cfr13I 753 GGACC G^G[ACGT]CC
DraII 752 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 621 GACACCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 423 TGGCCA [CT]^GGCC[GA]
EarI 403 GAAGAG CTCTTC(1/4)
EcoNI 604 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 752 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 438 CCTGG ^CC[AT]GG
EcoT22I 40 ATGCAT ATGCA^T
Fnu4HI 796 GCAGC GC^[ACGT]GC
FokI 38 GGATG GGATG(9/13)
HindIII 53 AAGCTT A^AGCTT
ItaI 796 GCAGC GC^[ACGT]GC
Ksp632I 403 GAAGAG CTCTTC(1/4)
MaeIII 504 GTTAC ^GT[ACGT]AC
MflI 733 AGATCT [GA]^GATC[CT]
MluNI 423 TGGCCA TGG^CCA
MscI 423 TGGCCA TGG^CCA
MvaI 438 CCTGG CC^[AT]GG
MwoI 601 GCACCTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 339 CATATG CA^TATG
NlaIII 35 CATG CATG^
NlaIV 752 GGGACC GG[ACGT]^[ACGT]CC
NsiI 40 ATGCAT ATGCA^T
PleI 355 GAGTC GAGTC(4/5)
PpuMI 752 GGGACCT [GA]G^G[AT]CC[CT]
Sau96I 753 GGACC G^G[ACGT]CC
ScrFI 438 CCTGG CC^[ACGT]GG
SmlI 590 CTCAAG C^T[CT][GA]AG
TseI 796 GCAGC G^C[AT]GC
TspRI 639 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 733 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AluI 54 AGCT AG^CT
281 AGCT
BsmFI 752 GGGAC GGGAC(10/14)
858 GGGAC
BsrI 681 CCAGT ACTGG(1/-1)
721 CCAGT
Cac8I 278 GCCAGC GC[ACGT]^[ACGT]GC
610 GCAGGC
HaeIII 190 GGCC GG^CC
424 GGCC
MfeI 273 CAATTG C^AATTG
419 CAATTG
MunI 273 CAATTG C^AATTG
419 CAATTG
TaqI 688 TCGA T^CGA
890 TCGA
TthHB8I 688 TCGA T^CGA
890 TCGA
XbaI 243 TCTAGA T^CTAGA
456 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AfaI ApoI BfaI BpmI
DdeI DpnII HinfI MaeI MboI
MboII MnlI MseI NdeII RsaI
Sau3AI SfaNI TfiI Tru9I Tsp509I