Restriction map for
pF1KE0527
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvrII BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BstAPI BstEII
BstPI BstXI Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MseI MslI MspA1I MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 43 CGCG CG^CG
AcyI 237 GACGCC G[GA]^CG[CT]C
AhdI 22 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 421 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 22 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 382 CCCGAG C^[CT]CG[GA]G
AviII 89 TGCGCA TGC^GCA
BalI 474 TGGCCA TGG^CCA
BamHI 725 GGATCC G^GATCC
BciVI 252 GTATCC GTATCC(6/5)
BfaI 7 CTAG C^TAG
BglI 694 GCCTATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 607 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 607 GCTGAGC GC^T[ACGT]AGC
BsaHI 237 GACGCC G[GA]^CG[CT]C
BsoBI 382 CCCGAG C^[CT]CG[GA]G
BspHI 285 TCATGA T^CATGA
BsrDI 429 GCAATG GCAATG(2/0)
BsrFI 525 GCCGGT [GA]^CCGG[CT]
BssSI 272 CTCGTG CACGAG(-5/-1)
Bst1107I 332 GTATAC GTA^TAC
BstBI 713 TTCGAA TT^CGAA
BstUI 43 CGCG CG^CG
BstYI 725 GGATCC [GA]^GATC[CT]
BstZ17I 332 GTATAC GTA^TAC
CelII 607 GCTGAGC GC^T[ACGT]AGC
Cfr10I 525 GCCGGT [GA]^CCGG[CT]
Eam1105I 22 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 648 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 413 CCATGG C^C[AT][AT]GG
FspI 89 TGCGCA TGC^GCA
Hin1I 237 GACGCC G[GA]^CG[CT]C
HinfI 716 GAATC G^A[ACGT]TC
MaeI 7 CTAG C^TAG
MaeIII 484 GTGAC ^GT[ACGT]AC
MflI 725 GGATCC [GA]^GATC[CT]
MluNI 474 TGGCCA TGG^CCA
MscI 474 TGGCCA TGG^CCA
MvnI 43 CGCG CG^CG
NcoI 413 CCATGG C^CATGG
NspI 303 GCATGC [GA]CATG^[CT]
NspV 713 TTCGAA TT^CGAA
PstI 265 CTGCAG CTGCA^G
RcaI 285 TCATGA T^CATGA
SbfI 264 CCTGCAGG CCTGCA^GG
SexAI 23 ACCTGGT A^CC[AT]GGT
SfcI 265 CTGCAG C^T[GA][CT]AG
SfuI 713 TTCGAA TT^CGAA
SphI 303 GCATGC GCATG^C
Sse8387I 264 CCTGCAGG CCTGCA^GG
StuI 692 AGGCCT AGG^CCT
StyI 413 CCATGG C^C[AT][AT]GG
TaqI 714 TCGA T^CGA
TfiI 716 GAATC G^A[AT]TC
Tsp45I 484 GTGAC ^GT[GC]AC
TthHB8I 714 TCGA T^CGA
XhoII 725 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 49 GTCTAC GT^[AC][GT]AC
332 GTATAC
AciI 42 CCGC CCGC(-3/-1)
658 GCGG
AfaI 231 GTAC GT^AC
291 GTAC
AlwI 726 GATCC GGATC(4/5)
725 GGATC
BanI 322 GGCACC G^G[CT][GA]CC
378 GGTGCC
BmyI 275 GTGCCC G[AGT]GC[ACT]^C
379 GTGCCC
BpmI 436 CTGGAG CTGGAG(16/14)
508 CTGGAG
BseRI 385 GAGGAG GAGGAG(10/8)
511 GAGGAG
Bsp1286I 275 GTGCCC G[AGT]GC[ACT]^C
379 GTGCCC
BspMI 263 ACCTGC ACCTGC(4/8)
480 GCAGGT
CfoI 44 GCGC GCG^C
90 GCGC
DraII 422 AGGCCCT [GA]G^G[ACGT]CC[CT]
460 AGGCCCT
EcoO109I 422 AGGCCCT [GA]G^G[ACGT]CC[CT]
460 AGGCCCT
HapII 241 CCGG C^CGG
526 CCGG
HgaI 165 GACGC GACGC(5/10)
237 GACGC
HhaI 44 GCGC GCG^C
90 GCGC
HinP1I 44 GCGC G^CGC
90 GCGC
HpaII 241 CCGG C^CGG
526 CCGG
MboII 66 GAAGA GAAGA(8/7)
56 TCTTC
MspI 241 CCGG C^CGG
526 CCGG
PflMI 348 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
361 CCACTCATTGG
RsaI 231 GTAC GT^AC
291 GTAC
SmlI 169 CTCAAG C^T[CT][GA]AG
639 CTTGAG
TspRI 247 CACTG CA[GC]TG[ACGT][ACGT]^
488 CACTG
Van91I 348 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
361 CCACTCATTGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII Fnu4HI FokI
HaeIII HphI ItaI MboI MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI