Restriction map for
pF1KE0148
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BseRI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeIII MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 584 ACATGT A^C[GA][CT]GT
AhdI 37 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 37 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 25 GAGCTC G[AT]GC[AT]^C
AvaI 353 CCCGGG C^[CT]CG[GA]G
BalI 573 TGGCCA TGG^CCA
BbsI 388 GAAGAC GAAGAC(2/6)
BpuAI 388 GAAGAC GAAGAC(2/6)
BsaBI 234 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 508 GAGACC GGTCTC(1/5)
BsaWI 602 ACCGGA [AT]^CCGG[AT]
BsiHKAI 25 GAGCTC G[AT]GC[AT]^C
BsmI 666 GAATGC GAATGC(1/-1)
BsoBI 353 CCCGGG C^[CT]CG[GA]G
BspHI 140 TCATGA T^CATGA
BspLU11I 584 ACATGT A^CATGT
BsrFI 80 GCCGGT [GA]^CCGG[CT]
BstYI 71 AGATCC [GA]^GATC[CT]
Cfr10I 80 GCCGGT [GA]^CCGG[CT]
EaeI 573 TGGCCA [CT]^GGCC[GA]
Eam1105I 37 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 28 CTCTTC CTCTTC(1/4)
EcoT14I 251 CCAAGG C^C[AT][AT]GG
FauI 179 CCCGC CCCGC(4/6)
HaeII 480 AGCGCC [GA]GCGC^[CT]
HinfI 234 GATTC G^A[ACGT]TC
Ksp632I 28 CTCTTC CTCTTC(1/4)
MaeII 38 ACGT A^CGT
MamI 234 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 71 AGATCC [GA]^GATC[CT]
MluNI 573 TGGCCA TGG^CCA
MscI 573 TGGCCA TGG^CCA
MseI 208 TTAA T^TAA
NspI 584 ACATGT [GA]CATG^[CT]
RcaI 140 TCATGA T^CATGA
SacI 25 GAGCTC GAGCT^C
SapI 27 GCTCTTC GCTCTTC(1/4)
SmaI 353 CCCGGG CCC^GGG
SmlI 502 CTCAAG C^T[CT][GA]AG
StyI 251 CCAAGG C^C[AT][AT]GG
TaqI 68 TCGA T^CGA
TfiI 234 GATTC G^A[AT]TC
Tru9I 208 TTAA T^TAA
TthHB8I 68 TCGA T^CGA
XcmI 582 CCACATGTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 71 AGATCC [GA]^GATC[CT]
XmaCI 353 CCCGGG C^CCGGG
XmaI 353 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 14 CGCG CG^CG
370 CGCG
AlwI 72 GATCC GGATC(4/5)
137 GGATC
ApaI 4 GGGCCC GGGCC^C
645 GGGCCC
BcnI 353 CCCGG CC^[GC]GG
354 CCGGG
BsmAI 219 GAGAC GTCTC(1/5)
508 GAGAC
BspMI 107 ACCTGC ACCTGC(4/8)
623 ACCTGC
BstUI 14 CGCG CG^CG
370 CGCG
BstXI 551 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCATGTGGATGG
Cac8I 523 GCCTGC GC[ACGT]^[ACGT]GC
615 GCTGGC
DdeI 217 CTGAG C^T[ACGT]AG
515 CTGAG
DsaI 20 CCGTGG C^C[GA][CT]GG
557 CCGTGG
HgaI 349 GCGTC GACGC(5/10)
371 GCGTC
MvnI 14 CGCG CG^CG
370 CGCG
NciI 353 CCCGG CC^[GC]GG
354 CCGGG
TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^
513 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI