Restriction map for
pF1KE0563
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I TspRI Tth111I XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcsI 97 AAATTT [GA]^AATT[CT]
AlwI 315 GATCC GGATC(4/5)
ApoI 97 AAATTT [GA]^AATT[CT]
Asp700I 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 513 CTCGAG C^[CT]CG[GA]G
BanII 266 GAGCCC G[GA]GC[CT]^C
BfaI 103 CTAG C^TAG
BlpI 456 GCTGAGC GC^T[ACGT]AGC
BmyI 266 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BsaAI 496 TACGTG [CT]AC^GT[GA]
BsgI 196 GTGCAG GTGCAG(16/14)
BsoBI 513 CTCGAG C^[CT]CG[GA]G
Bsp1286I 266 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 281 TGTACA T^GTACA
BspMI 569 ACCTGC ACCTGC(4/8)
BsrDI 222 CATTGC GCAATG(2/0)
BsrGI 281 TGTACA T^GTACA
Cac8I 226 GCCAGC GC[ACGT]^[ACGT]GC
CelII 456 GCTGAGC GC^T[ACGT]AGC
Cfr13I 546 GGCCC G^G[ACGT]CC
DdeI 457 CTGAG C^T[ACGT]AG
DpnII 315 GATC ^GATC
DraII 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 475 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeIII 546 GGCC GG^CC
HincII 335 GTCAAC GT[CT]^[GA]AC
HindII 335 GTCAAC GT[CT]^[GA]AC
MaeI 103 CTAG C^TAG
MaeII 497 ACGT A^CGT
MboI 315 GATC ^GATC
MspA1I 74 CAGCTG C[AC]G^C[GT]G
NcoI 620 CCATGG C^CATGG
NdeI 330 CATATG CA^TATG
NdeII 315 GATC ^GATC
NlaIV 62 GGCTCC GG[ACGT]^[ACGT]CC
PaeR7I 513 CTCGAG C^TCGAG
PflMI 53 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 511 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 74 CAGCTG CAG^CTG
SapI 502 GAAGAGC GCTCTTC(1/4)
Sau3AI 315 GATC ^GATC
Sau96I 546 GGCCC G^G[ACGT]CC
SexAI 476 ACCTGGT A^CC[AT]GGT
SfaNI 352 GATGC GCATC(5/9)
SmlI 513 CTCGAG C^T[CT][GA]AG
SspBI 281 TGTACA T^GTACA
TaqI 514 TCGA T^CGA
Tsp509I 98 AATT ^AATT
TthHB8I 514 TCGA T^CGA
Van91I 53 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 102 TCTAGA T^CTAGA
XcmI 356 CCAAGACTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 513 CTCGAG C^TCGAG
XmnI 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 163 GTAC GT^AC
282 GTAC
BpmI 525 CTCCAG CTGGAG(16/14)
77 CTGGAG
BstF5I 185 GGATG GGATG(2/0)
624 GGATG
DsaI 575 CCACGG C^C[GA][CT]GG
620 CCATGG
EarI 91 GAAGAG CTCTTC(1/4)
502 GAAGAG
EcoT14I 365 CCTTGG C^C[AT][AT]GG
620 CCATGG
FokI 185 GGATG GGATG(9/13)
624 GGATG
HphI 579 GGTGA GGTGA(8/7)
474 TCACC
Ksp632I 91 GAAGAG CTCTTC(1/4)
502 GAAGAG
MwoI 150 GCTGTTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
355 GCCAAGACTGC
NlaIII 255 CATG CATG^
621 CATG
RsaI 163 GTAC GT^AC
282 GTAC
StyI 365 CCTTGG C^C[AT][AT]GG
620 CCATGG
TfiI 106 GAATC G^A[AT]TC
560 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstNI EcoRII Fnu4HI HinfI
ItaI MboII MnlI MseI MvaI
PleI PstI ScrFI SfcI Tru9I
TseI