Restriction map for
pF1KB7350
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AvrII BciVI BfrI
BlnI BsaBI BsiEI BspDI BspHI
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FseI HpaI KpnI MamI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 2292 TCCGGA T^CCGGA
AcsI 525 GAATTT [GA]^AATT[CT]
AflIII 902 ACATGT A^C[GA][CT]GT
AhdI 1690 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 525 GAATTT [GA]^AATT[CT]
Asp700I 3190 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1690 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3092 TGCGCA TGC^GCA
BamHI 710 GGATCC G^GATCC
BbeI 2482 GGCGCC GGCGC^C
BbrPI 1339 CACGTG CAC^GTG
BcgI 1742 CGACTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2912 TGATCA T^GATCA
BglI 542 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2408 AGATCT A^GATCT
BsaWI 2292 TCCGGA [AT]^CCGG[AT]
BseAI 2292 TCCGGA T^CCGGA
BsgI 1431 CTGCAC GTGCAG(16/14)
BsiWI 1205 CGTACG C^GTACG
BsmBI 894 CGTCTC CGTCTC(1/5)
Bsp1407I 381 TGTACA T^GTACA
BspEI 2292 TCCGGA T^CCGGA
BspLU11I 902 ACATGT A^CATGT
BsrFI 1416 GCCGGT [GA]^CCGG[CT]
BsrGI 381 TGTACA T^GTACA
BstAPI 1775 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1416 GCCGGT [GA]^CCGG[CT]
DrdI 849 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1690 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2912 TGATCA T^GATCA
FspI 3092 TGCGCA TGC^GCA
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
KasI 2482 GGCGCC G^GCGCC
KspI 1100 CCGCGG CCGC^GG
MroI 2292 TCCGGA T^CCGGA
NarI 2482 GGCGCC GG^CGCC
PmaCI 1339 CACGTG CAC^GTG
PmlI 1339 CACGTG CAC^GTG
SacII 1100 CCGCGG CCGC^GG
SexAI 1693 ACCAGGT A^CC[AT]GGT
SfoI 2482 GGCGCC GGC^GCC
SphI 1771 GCATGC GCATG^C
SspBI 381 TGTACA T^GTACA
StuI 469 AGGCCT AGG^CCT
Tsp509I 526 AATT ^AATT
XmnI 3190 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1101 CGCG CG^CG
3109 CGCG
Aor51HI 363 AGCGCT AGC^GCT
1181 AGCGCT
ApaI 251 GGGCCC GGGCC^C
2503 GGGCCC
AvaI 649 CCCGGG C^[CT]CG[GA]G
706 CCCGGG
BsmI 2746 GAATGC GAATGC(1/-1)
3194 GCATTC
BsoBI 649 CCCGGG C^[CT]CG[GA]G
706 CCCGGG
BsrBI 2303 CCGCTC CCGCTC(-3/-3)
2515 CCGCTC
BstEII 56 GGTCACC G^GT[ACGT]ACC
2077 GGTGACC
BstPI 56 GGTCACC G^GT[ACGT]ACC
2077 GGTGACC
BstUI 1101 CGCG CG^CG
3109 CGCG
Eco47III 363 AGCGCT AGC^GCT
1181 AGCGCT
EcoO65I 56 GGTCACC G^GT[ACGT]ACC
2077 GGTGACC
FauI 2302 CCCGC CCCGC(4/6)
2514 CCCGC
HindIII 1130 AAGCTT A^AGCTT
3086 AAGCTT
MvnI 1101 CGCG CG^CG
3109 CGCG
NcoI 2834 CCATGG C^CATGG
3199 CCATGG
SmaI 649 CCCGGG CCC^GGG
706 CCCGGG
SmlI 2321 CTCAAG C^T[CT][GA]AG
2720 CTCAAG
XmaCI 649 CCCGGG C^CCGGG
706 CCCGGG
XmaI 649 CCCGGG C^CCGGG
706 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AspI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I XcmI
XhoII