Restriction map for
pF1KB4372
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglII BlnI BsaAI
BsaHI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI HaeII Hin1I HindIII
KasI KpnI KspI MflI MluI
MroI MvnI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 649 GTCTAC GT^[AC][GT]AC
AlwI 342 GATCC GGATC(4/5)
AlwNI 178 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 415 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 47 GTGCTC G[AT]GC[AT]^C
AviII 890 TGCGCA TGC^GCA
BalI 1523 TGGCCA TGG^CCA
BbsI 762 GAAGAC GAAGAC(2/6)
BbvCI 582 CCTCAGC CCTCAGC(-5/-2)
BglI 1525 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 175 GCTCAGC GC^T[ACGT]AGC
BpmI 619 CTCCAG CTGGAG(16/14)
Bpu1102I 175 GCTCAGC GC^T[ACGT]AGC
BpuAI 762 GAAGAC GAAGAC(2/6)
BsaBI 1044 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1049 CGATCG CG[GA][CT]^CG
BsiHKAI 47 GTGCTC G[AT]GC[AT]^C
BsrBI 795 GAGCGG CCGCTC(-3/-3)
BsrFI 2096 GCCGGC [GA]^CCGG[CT]
BstXI 731 CCAATTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 175 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2096 GCCGGC [GA]^CCGG[CT]
DraI 1368 TTTAAA TTT^AAA
DraII 870 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1523 TGGCCA [CT]^GGCC[GA]
EcoO109I 870 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 345 CCATGG C^C[AT][AT]GG
FspI 890 TGCGCA TGC^GCA
HapII 2097 CCGG C^CGG
HgaI 910 GACGC GACGC(5/10)
HpaII 2097 CCGG C^CGG
MamI 1044 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1523 TGGCCA TGG^CCA
MscI 1523 TGGCCA TGG^CCA
MspA1I 2056 CAGCTG C[AC]G^C[GT]G
MspI 2097 CCGG C^CGG
NaeI 2096 GCCGGC GCC^GGC
NcoI 345 CCATGG C^CATGG
NgoMIV 2096 GCCGGC G^CCGGC
NspI 789 GCATGT [GA]CATG^[CT]
PflMI 931 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 883 GACTC GAGTC(4/5)
PpuMI 870 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 1049 CGATCG CGAT^CG
PvuII 2056 CAGCTG CAG^CTG
SmlI 757 CTCAAG C^T[CT][GA]AG
StuI 1511 AGGCCT AGG^CCT
StyI 345 CCATGG C^C[AT][AT]GG
Van91I 931 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 415 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 1493 CCGC CCGC(-3/-1)
797 GCGG
AfaI 1570 GTAC GT^AC
1664 GTAC
BanII 596 GAGCCC G[GA]GC[CT]^C
1425 GAGCCC
BfaI 256 CTAG C^TAG
442 CTAG
BsgI 1635 CTGCAC GTGCAG(16/14)
1450 GTGCAG
BspHI 377 TCATGA T^CATGA
423 TCATGA
BspMI 437 ACCTGC ACCTGC(4/8)
1297 GCAGGT
BsrDI 470 CATTGC GCAATG(2/0)
693 CATTGC
DsaI 345 CCATGG C^C[GA][CT]GG
866 CCACGG
HincII 148 GTTAAC GT[CT]^[GA]AC
1833 GTTAAC
HindII 148 GTTAAC GT[CT]^[GA]AC
1833 GTTAAC
HpaI 148 GTTAAC GTT^AAC
1833 GTTAAC
MaeI 256 CTAG C^TAG
442 CTAG
MfeI 558 CAATTG C^AATTG
644 CAATTG
MunI 558 CAATTG C^AATTG
644 CAATTG
RcaI 377 TCATGA T^CATGA
423 TCATGA
RsaI 1570 GTAC GT^AC
1664 GTAC
TaqI 1821 TCGA T^CGA
2135 TCGA
Tsp45I 265 GTCAC ^GT[GC]AC
1540 GTGAC
TthHB8I 1821 TCGA T^CGA
2135 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoRV Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI XcmI