Restriction map for pF1KE0002
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco81I      EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       ScaI        SexAI       
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1409  GTCGAC               GT^[AC][GT]AC
AcsI          316  AAATTT               [GA]^AATT[CT]
AflII         724  CTTAAG               C^TTAAG
AlwI          903  GATCC                GGATC(4/5)
Aor51HI       720  AGCGCT               AGC^GCT
ApoI          316  AAATTT               [GA]^AATT[CT]
BbeI         1278  GGCGCC               GGCGC^C
BclI          230  TGATCA               T^GATCA
BfaI         1589  CTAG                 C^TAG
BfrI          724  CTTAAG               C^TTAAG
BglI         1566  GCCTCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         968  AGATCT               A^GATCT
BsaWI        1291  ACCGGA               [AT]^CCGG[AT]
BsmBI         766  CGTCTC               CGTCTC(1/5)
BsrBI        1129  GAGCGG               CCGCTC(-3/-3)
BstYI         968  AGATCT               [GA]^GATC[CT]
DrdI          945  GACGGCCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1430  CCACGG               C^C[GA][CT]GG
Eco47III      720  AGCGCT               AGC^GCT
EcoNI         261  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          230  TGATCA               T^GATCA
KasI         1278  GGCGCC               G^GCGCC
MaeI         1589  CTAG                 C^TAG
MaeII         833  ACGT                 A^CGT
MflI          968  AGATCT               [GA]^GATC[CT]
MslI          957  CATTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1100  GCCGGC               GCC^GGC
NarI         1278  GGCGCC               GG^CGCC
NgoMIV       1100  GCCGGC               G^CCGGC
NlaIII       1251  CATG                 CATG^
PpuMI         794  GGGTCCC              [GA]G^G[AT]CC[CT]
SalI         1409  GTCGAC               G^TCGAC
SapI          372  GCTCTTC              GCTCTTC(1/4)
SbfI          990  CCTGCAGG             CCTGCA^GG
SfiI         1565  GGCCTCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1278  GGCGCC               GGC^GCC
Sse8387I      990  CCTGCAGG             CCTGCA^GG
StuI          258  AGGCCT               AGG^CCT
TfiI          460  GAATC                G^A[AT]TC
Tsp509I       317  AATT                 ^AATT
XhoII         968  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BsaI          100  GAGACC               GGTCTC(1/5)
              895  GGTCTC              
BstEII         81  GGTCACC              G^GT[ACGT]ACC
             1116  GGTGACC             
BstPI          81  GGTCACC              G^GT[ACGT]ACC
             1116  GGTGACC             
EarI          373  CTCTTC               CTCTTC(1/4)
             1090  GAAGAG              
EcoO65I        81  GGTCACC              G^GT[ACGT]ACC
             1116  GGTGACC             
HaeII         720  AGCGCT               [GA]GCGC^[CT]
             1278  GGCGCC              
HincII       1351  GTCAAC               GT[CT]^[GA]AC
             1409  GTCGAC              
HindII       1351  GTCAAC               GT[CT]^[GA]AC
             1409  GTCGAC              
Ksp632I       373  CTCTTC               CTCTTC(1/4)
             1090  GAAGAG              
MaeIII         82  GTCAC                ^GT[ACGT]AC
             1117  GTGAC               
MseI          136  TTAA                 T^TAA
              725  TTAA                
SacI         1177  GAGCTC               GAGCT^C
             1363  GAGCTC              
SmlI          724  CTTAAG               C^T[CT][GA]AG
             1366  CTCAAG              
Tru9I         136  TTAA                 T^TAA
              725  TTAA                
Tsp45I         82  GTCAC                ^GT[GC]AC
             1117  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        TaqI        TseI        TspRI       TthHB8I     
XmaCI       XmaI        
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