################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1409 GTCGAC GT^[AC][GT]AC AcsI 316 AAATTT [GA]^AATT[CT] AflII 724 CTTAAG C^TTAAG AlwI 903 GATCC GGATC(4/5) Aor51HI 720 AGCGCT AGC^GCT ApoI 316 AAATTT [GA]^AATT[CT] BbeI 1278 GGCGCC GGCGC^C BclI 230 TGATCA T^GATCA BfaI 1589 CTAG C^TAG BfrI 724 CTTAAG C^TTAAG BglI 1566 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 968 AGATCT A^GATCT BsaWI 1291 ACCGGA [AT]^CCGG[AT] BsmBI 766 CGTCTC CGTCTC(1/5) BsrBI 1129 GAGCGG CCGCTC(-3/-3) BstYI 968 AGATCT [GA]^GATC[CT] DrdI 945 GACGGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1430 CCACGG C^C[GA][CT]GG Eco47III 720 AGCGCT AGC^GCT EcoNI 261 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 230 TGATCA T^GATCA KasI 1278 GGCGCC G^GCGCC MaeI 1589 CTAG C^TAG MaeII 833 ACGT A^CGT MflI 968 AGATCT [GA]^GATC[CT] MslI 957 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1100 GCCGGC GCC^GGC NarI 1278 GGCGCC GG^CGCC NgoMIV 1100 GCCGGC G^CCGGC NlaIII 1251 CATG CATG^ PpuMI 794 GGGTCCC [GA]G^G[AT]CC[CT] SalI 1409 GTCGAC G^TCGAC SapI 372 GCTCTTC GCTCTTC(1/4) SbfI 990 CCTGCAGG CCTGCA^GG SfiI 1565 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1278 GGCGCC GGC^GCC Sse8387I 990 CCTGCAGG CCTGCA^GG StuI 258 AGGCCT AGG^CCT TfiI 460 GAATC G^A[AT]TC Tsp509I 317 AATT ^AATT XhoII 968 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BsaI 100 GAGACC GGTCTC(1/5) 895 GGTCTC BstEII 81 GGTCACC G^GT[ACGT]ACC 1116 GGTGACC BstPI 81 GGTCACC G^GT[ACGT]ACC 1116 GGTGACC EarI 373 CTCTTC CTCTTC(1/4) 1090 GAAGAG EcoO65I 81 GGTCACC G^GT[ACGT]ACC 1116 GGTGACC HaeII 720 AGCGCT [GA]GCGC^[CT] 1278 GGCGCC HincII 1351 GTCAAC GT[CT]^[GA]AC 1409 GTCGAC HindII 1351 GTCAAC GT[CT]^[GA]AC 1409 GTCGAC Ksp632I 373 CTCTTC CTCTTC(1/4) 1090 GAAGAG MaeIII 82 GTCAC ^GT[ACGT]AC 1117 GTGAC MseI 136 TTAA T^TAA 725 TTAA SacI 1177 GAGCTC GAGCT^C 1363 GAGCTC SmlI 724 CTTAAG C^T[CT][GA]AG 1366 CTCAAG Tru9I 136 TTAA T^TAA 725 TTAA Tsp45I 82 GTCAC ^GT[GC]AC 1117 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI TspRI TthHB8I XmaCI XmaI