##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI           69  GCGG                 CCGC(-3/-1)
AfaI           51  GTAC                 GT^AC
AvaI           94  CCCGAG               C^[CT]CG[GA]G
AvaII         132  GGACC                G^G[AT]CC
BamHI         122  GGATCC               G^GATCC
BbvI           99  GCTGC                GCAGC(8/12)
BseRI           4  GAGGAG               GAGGAG(10/8)
BsmFI          11  GGGAC                GGGAC(10/14)
BsoBI          94  CCCGAG               C^[CT]CG[GA]G
BstYI         122  GGATCC               [GA]^GATC[CT]
Cac8I          57  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        132  GGACC                G^G[ACGT]CC
DraII         131  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          169  CCATGG               C^C[GA][CT]GG
EcoO109I      131  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI           69  GCGGG                CCCGC(4/6)
Fnu4HI         99  GCTGC                GC^[ACGT]GC
HaeIII         60  GGCC                 GG^CC
ItaI           99  GCTGC                GC^[ACGT]GC
MaeII          14  ACGT                 A^CGT
MflI          122  GGATCC               [GA]^GATC[CT]
NcoI          169  CCATGG               C^CATGG
NlaIII        170  CATG                 CATG^
PpuMI         131  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII          55  CAGCTG               CAG^CTG
RsaI           51  GTAC                 GT^AC
Sau96I        132  GGACC                G^G[ACGT]CC
SmlI           46  CTCAAG               C^T[CT][GA]AG
TaqI          113  TCGA                 T^CGA
TseI           99  GCTGC                G^C[AT]GC
TthHB8I       113  TCGA                 T^CGA
XhoII         122  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI           56  AGCT                 AG^CT
               98  AGCT                
BsaJI         128  CCAAGG               C^C[ACGT][ACGT]GG
              169  CCATGG              
BsiYI          62  CCTTCCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              168  CCCATGGGTGG         
BslI           62  CCTTCCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              168  CCCATGGGTGG         
BstF5I         90  CATCC                GGATG(2/0)
              195  CATCC               
DpnII         111  GATC                 ^GATC
              123  GATC                
EcoT14I       128  CCAAGG               C^C[AT][AT]GG
              169  CCATGG              
FokI           90  CATCC                GGATG(9/13)
              195  CATCC               
MboI          111  GATC                 ^GATC
              123  GATC                
MboII          27  GAAGA                GAAGA(8/7)
              159  GAAGA               
MspA1I         55  CAGCTG               C[AC]G^C[GT]G
               67  CAGCGG              
NdeII         111  GATC                 ^GATC
              123  GATC                
NlaIV         122  GGATCC               GG[ACGT]^[ACGT]CC
              132  GGACCC              
Sau3AI        111  GATC                 ^GATC
              123  GATC                
StyI          128  CCAAGG               C^C[AT][AT]GG
              169  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AlwI        MnlI