Restriction map for
pF1KE0353
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 937 GTAGAC GT^[AC][GT]AC
AcsI 1618 AAATTC [GA]^AATT[CT]
AocI 1181 CCTCAGG CC^T[ACGT]AGG
ApoI 1618 AAATTC [GA]^AATT[CT]
Asp700I 685 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1170 GGACC G^G[AT]CC
BamHI 428 GGATCC G^GATCC
BanII 344 GAGCCC G[GA]GC[CT]^C
BbvCI 121 GCTGAGG CCTCAGC(-5/-2)
BcgI 867 GCAAGTCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 604 GTATCC GTATCC(6/5)
BclI 221 TGATCA T^GATCA
BcnI 167 CCCGG CC^[GC]GG
BsgI 1327 CTGCAC GTGCAG(16/14)
BspHI 512 TCATGA T^CATGA
BstXI 584 CCAATGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1181 CCTCAGG CC^T[ACGT]AGG
Eco81I 1181 CCTCAGG CC^T[ACGT]AGG
EcoNI 442 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 922 CCAAGG C^C[AT][AT]GG
EcoT22I 203 ATGCAT ATGCA^T
FbaI 221 TGATCA T^GATCA
HapII 168 CCGG C^CGG
HincII 1546 GTTGAC GT[CT]^[GA]AC
HindII 1546 GTTGAC GT[CT]^[GA]AC
HpaII 168 CCGG C^CGG
MslI 246 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 168 CCGG C^CGG
NciI 167 CCCGG CC^[GC]GG
NsiI 203 ATGCAT ATGCA^T
PleI 1336 GACTC GAGTC(4/5)
PpuMI 1169 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 512 TCATGA T^CATGA
SapI 818 GCTCTTC GCTCTTC(1/4)
StyI 922 CCAAGG C^C[AT][AT]GG
XmnI 685 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 589 GCGG CCGC(-3/-1)
986 GCGG
BbsI 621 GAAGAC GAAGAC(2/6)
255 GTCTTC
BmyI 344 GAGCCC G[AGT]GC[ACT]^C
1423 GGGCAC
BpuAI 621 GAAGAC GAAGAC(2/6)
255 GTCTTC
BsmAI 1220 GAGAC GTCTC(1/5)
1092 GTCTC
BsmI 98 GAATGC GAATGC(1/-1)
205 GCATTC
Bsp1286I 344 GAGCCC G[AGT]GC[ACT]^C
1423 GGGCAC
BsrDI 651 CATTGC GCAATG(2/0)
824 CATTGC
HphI 1186 GGTGA GGTGA(8/7)
1402 TCACC
MseI 881 TTAA T^TAA
1616 TTAA
PstI 1078 CTGCAG CTGCA^G
1215 CTGCAG
SfcI 1078 CTGCAG C^T[GA][CT]AG
1215 CTGCAG
SspI 687 AATATT AAT^ATT
788 AATATT
TaqI 876 TCGA T^CGA
1603 TCGA
Tru9I 881 TTAA T^TAA
1616 TTAA
TthHB8I 876 TCGA T^CGA
1603 TCGA
XcmI 488 CCAGATCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1062 CCATTCCTCATTTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI AlwI AlwNI BbvI
BfaI BglII BsaJI BseRI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
Tsp509I TspRI XhoII