##################################
# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MaeIII
MamI MboI MfeI MflI MluI
MroI MseI MslI MspA1I MunI
MvnI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 168 CCGC CCGC(-3/-1)
AfaI 267 GTAC GT^AC
AlwNI 283 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 149 GAGCTC G[AT]GC[AT]^C
AvaI 146 CCCGAG C^[CT]CG[GA]G
AvaII 14 GGACC G^G[AT]CC
BalI 59 TGGCCA TGG^CCA
BcnI 171 CCCGG CC^[GC]GG
BfaI 154 CTAG C^TAG
BglI 325 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 72 CTCCAG CTGGAG(16/14)
BsaWI 218 TCCGGT [AT]^CCGG[AT]
BseRI 34 CTCCTC GAGGAG(10/8)
BsiHKAI 149 GAGCTC G[AT]GC[AT]^C
BsmFI 13 GGGAC GGGAC(10/14)
BsoBI 146 CCCGAG C^[CT]CG[GA]G
BsrI 210 ACTGG ACTGG(1/-1)
DdeI 82 CTGAG C^T[ACGT]AG
DsaI 62 CCATGG C^C[GA][CT]GG
EaeI 59 TGGCCA [CT]^GGCC[GA]
EarI 319 GAAGAG CTCTTC(1/4)
EcoT14I 62 CCATGG C^C[AT][AT]GG
Ksp632I 319 GAAGAG CTCTTC(1/4)
MaeI 154 CTAG C^TAG
MluNI 59 TGGCCA TGG^CCA
MscI 59 TGGCCA TGG^CCA
NciI 171 CCCGG CC^[GC]GG
NcoI 62 CCATGG C^CATGG
NspI 41 GCATGC [GA]CATG^[CT]
PflMI 282 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 267 GTAC GT^AC
SacI 149 GAGCTC GAGCT^C
SfiI 324 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 41 GCATGC GCATG^C
StyI 62 CCATGG C^C[AT][AT]GG
TaqI 118 TCGA T^CGA
TfiI 245 GAATC G^A[AT]TC
TthHB8I 118 TCGA T^CGA
Van91I 282 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BanII 84 GAGCCC G[GA]GC[CT]^C
149 GAGCTC
BmyI 84 GAGCCC G[AGT]GC[ACT]^C
149 GAGCTC
Bsp1286I 84 GAGCCC G[AGT]GC[ACT]^C
149 GAGCTC
BstF5I 309 CATCC GGATG(2/0)
204 GGATG
DraII 284 AGGCCCT [GA]G^G[ACGT]CC[CT]
332 AGGCCCC
EcoO109I 284 AGGCCCT [GA]G^G[ACGT]CC[CT]
332 AGGCCCC
FokI 309 CATCC GGATG(9/13)
204 GGATG
HapII 172 CCGG C^CGG
219 CCGG
HpaII 172 CCGG C^CGG
219 CCGG
MspI 172 CCGG C^CGG
219 CCGG
NlaIII 42 CATG CATG^
63 CATG
PleI 101 GAGTC GAGTC(4/5)
115 GAGTC
PstI 21 CTGCAG CTGCA^G
139 CTGCAG
SfcI 21 CTGCAG C^T[GA][CT]AG
139 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI HaeIII HinfI ItaI MboII
MnlI MvaI MwoI NlaIV Sau96I
ScrFI TseI