Restriction map for
pF1KE0345
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaAI BsaBI BsaHI BsiWI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI MaeI MamI MfeI MluI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacI SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1302 GGTACC G^GTACC
AccIII 1394 TCCGGA T^CCGGA
Asp718I 1302 GGTACC G^GTACC
BamHI 1241 GGATCC G^GATCC
BglI 1379 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1070 AGATCT A^GATCT
BsaI 1582 GAGACC GGTCTC(1/5)
BseAI 1394 TCCGGA T^CCGGA
BseRI 1498 GAGGAG GAGGAG(10/8)
BsiEI 728 CGGCCG CG[GA][CT]^CG
BsmI 234 GAATGC GAATGC(1/-1)
BspEI 1394 TCCGGA T^CCGGA
BspHI 230 TCATGA T^CATGA
BspMI 1314 ACCTGC ACCTGC(4/8)
BsrDI 204 CATTGC GCAATG(2/0)
BsrFI 1699 GCCGGC [GA]^CCGG[CT]
CfoI 835 GCGC GCG^C
Cfr10I 1699 GCCGGC [GA]^CCGG[CT]
CpoI 544 CGGACCG CG^G[AT]CCG
DrdI 1384 GACGGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 728 CGGCCG C^GGCCG
EclXI 728 CGGCCG C^GGCCG
Eco52I 728 CGGCCG C^GGCCG
HhaI 835 GCGC GCG^C
HinP1I 835 GCGC G^CGC
HinfI 1675 GACTC G^A[ACGT]TC
KpnI 1302 GGTACC GGTAC^C
KspI 131 CCGCGG CCGC^GG
MroI 1394 TCCGGA T^CCGGA
NaeI 1699 GCCGGC GCC^GGC
NgoMIV 1699 GCCGGC G^CCGGC
NspI 824 ACATGC [GA]CATG^[CT]
PflMI 240 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1675 GACTC GAGTC(4/5)
PstI 1571 CTGCAG CTGCA^G
RcaI 230 TCATGA T^CATGA
RsrII 544 CGGACCG CG^G[AT]CCG
SacII 131 CCGCGG CCGC^GG
SfcI 1571 CTGCAG C^T[GA][CT]AG
SfiI 1378 GGCCAGGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 896 CCCGGG CCC^GGG
StuI 677 AGGCCT AGG^CCT
Tsp509I 1321 AATT ^AATT
Van91I 240 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 896 CCCGGG C^CCGGG
XmaI 896 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 1519 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1565 CAGTGACTG
ApaI 847 GGGCCC GGGCC^C
1064 GGGCCC
AspHI 147 GTGCTC G[AT]GC[AT]^C
784 GAGCAC
BalI 659 TGGCCA TGG^CCA
1448 TGGCCA
BcgI 245 GCATGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1663 GCAGCTGCCTCG
BlpI 772 GCTGAGC GC^T[ACGT]AGC
840 GCTCAGC
BpmI 1677 CTCCAG CTGGAG(16/14)
1579 CTGGAG
Bpu1102I 772 GCTGAGC GC^T[ACGT]AGC
840 GCTCAGC
BsaWI 959 TCCGGT [AT]^CCGG[AT]
1394 TCCGGA
BsgI 1442 CTGCAC GTGCAG(16/14)
1510 CTGCAC
BsiHKAI 147 GTGCTC G[AT]GC[AT]^C
784 GAGCAC
BsmBI 652 GAGACG CGTCTC(1/5)
809 GAGACG
Bsp1407I 110 TGTACA T^GTACA
821 TGTACA
BsrGI 110 TGTACA T^GTACA
821 TGTACA
BsrI 1446 ACTGG ACTGG(1/-1)
830 CCAGT
BstAPI 199 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1564 GCAGTGACTGC
CelII 772 GCTGAGC GC^T[ACGT]AGC
840 GCTCAGC
EarI 265 CTCTTC CTCTTC(1/4)
466 CTCTTC
EcoT14I 89 CCAAGG C^C[AT][AT]GG
1217 CCAAGG
HgaI 654 GACGC GACGC(5/10)
1609 GCGTC
Ksp632I 265 CTCTTC CTCTTC(1/4)
466 CTCTTC
MluNI 659 TGGCCA TGG^CCA
1448 TGGCCA
MscI 659 TGGCCA TGG^CCA
1448 TGGCCA
MslI 1536 CACGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1620 CATCGCTGTG
SspBI 110 TGTACA T^GTACA
821 TGTACA
StyI 89 CCAAGG C^C[AT][AT]GG
1217 CCAAGG
XcmI 116 CCAGGGCTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
476 CCAGCATCCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I XhoII