Restriction map for
pF1KA0219
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# NO CUT ENZYMES ( 54 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI BbeI
BfrI BsiWI Bst1107I BstBI BstZ17I
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FseI KasI KpnI KspI
MluI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacII SexAI SfoI SfuI SnaBI
SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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ApaI 5326 GGGCCC GGGCC^C
AviII 371 TGCGCA TGC^GCA
AvrII 7347 CCTAGG C^CTAGG
BciVI 5342 GTATCC GTATCC(6/5)
BclI 2012 TGATCA T^GATCA
BlnI 7347 CCTAGG C^CTAGG
BsiEI 3631 CGGCCG CG[GA][CT]^CG
BsmBI 7281 CGTCTC CGTCTC(1/5)
Bsp1407I 5666 TGTACA T^GTACA
BspLU11I 4922 ACATGT A^CATGT
BsrGI 5666 TGTACA T^GTACA
BssHII 5394 GCGCGC G^CGCGC
BstEII 913 GGTCACC G^GT[ACGT]ACC
BstPI 913 GGTCACC G^GT[ACGT]ACC
EagI 3631 CGGCCG C^GGCCG
EclXI 3631 CGGCCG C^GGCCG
Eco52I 3631 CGGCCG C^GGCCG
EcoO65I 913 GGTCACC G^GT[ACGT]ACC
FbaI 2012 TGATCA T^GATCA
FspI 371 TGCGCA TGC^GCA
HpaI 7939 GTTAAC GTT^AAC
PaeR7I 6979 CTCGAG C^TCGAG
SacI 5124 GAGCTC GAGCT^C
SalI 2641 GTCGAC G^TCGAC
ScaI 545 AGTACT AGT^ACT
SgrAI 4297 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 5666 TGTACA T^GTACA
XhoI 6979 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 2641 GTCGAC GT^[AC][GT]AC
4604 GTCTAC
AcsI 769 GAATTT [GA]^AATT[CT]
1585 AAATTC
ApoI 769 GAATTT [GA]^AATT[CT]
1585 AAATTC
AspI 640 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2206 GACCGGGTC
BamHI 4758 GGATCC G^GATCC
7562 GGATCC
BcgI 1461 CGAAGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1755 GCAGACAGTTCG
BglII 120 AGATCT A^GATCT
5850 AGATCT
BsaI 5918 GGTCTC GGTCTC(1/5)
6579 GGTCTC
BsmI 2172 GAATGC GAATGC(1/-1)
6096 GCATTC
BspDI 176 ATCGAT AT^CGAT
2086 ATCGAT
ClaI 176 ATCGAT AT^CGAT
2086 ATCGAT
CpoI 2204 CGGACCG CG^G[AT]CCG
5020 CGGACCG
MfeI 6402 CAATTG C^AATTG
7844 CAATTG
MunI 6402 CAATTG C^AATTG
7844 CAATTG
PflFI 640 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2206 GACCGGGTC
RsrII 2204 CGGACCG CG^G[AT]CCG
5020 CGGACCG
SbfI 1926 CCTGCAGG CCTGCA^GG
7065 CCTGCAGG
Sse8387I 1926 CCTGCAGG CCTGCA^GG
7065 CCTGCAGG
Tth111I 640 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2206 GACCGGGTC
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# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI