Restriction map for
pF1KB6140
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BcgI BciVI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DrdI Eco81I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 1848 GTAC GT^AC
AhdI 271 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 539 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 39 AGCGCT AGC^GCT
ApaI 241 GGGCCC GGGCC^C
AspEI 271 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 206 TGGCCA TGG^CCA
BbeI 1303 GGCGCC GGCGC^C
BclI 431 TGATCA T^GATCA
BlpI 751 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 751 GCTCAGC GC^T[ACGT]AGC
BsaI 288 GAGACC GGTCTC(1/5)
BspHI 398 TCATGA T^CATGA
BspLU11I 1247 ACATGT A^CATGT
BssSI 475 CTCGTG CACGAG(-5/-1)
BstEII 1002 GGTCACC G^GT[ACGT]ACC
BstPI 1002 GGTCACC G^GT[ACGT]ACC
BstYI 1085 AGATCC [GA]^GATC[CT]
CelII 751 GCTCAGC GC^T[ACGT]AGC
DraI 117 TTTAAA TTT^AAA
Eam1105I 271 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 39 AGCGCT AGC^GCT
EcoO65I 1002 GGTCACC G^GT[ACGT]ACC
FbaI 431 TGATCA T^GATCA
HincII 910 GTCAAC GT[CT]^[GA]AC
HindII 910 GTCAAC GT[CT]^[GA]AC
KasI 1303 GGCGCC G^GCGCC
MflI 1085 AGATCC [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MseI 118 TTAA T^TAA
NarI 1303 GGCGCC GG^CGCC
NotI 1036 GCGGCCGC GC^GGCCGC
RcaI 398 TCATGA T^CATGA
RsaI 1848 GTAC GT^AC
SapI 1296 GAAGAGC GCTCTTC(1/4)
SexAI 1157 ACCTGGT A^CC[AT]GGT
SfoI 1303 GGCGCC GGC^GCC
SpeI 985 ACTAGT A^CTAGT
SphI 13 GCATGC GCATG^C
StuI 1577 AGGCCT AGG^CCT
TfiI 840 GATTC G^A[AT]TC
Tru9I 118 TTAA T^TAA
Tsp509I 452 AATT ^AATT
XhoII 1085 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 877 CGCG CG^CG
1549 CGCG
AflIII 1247 ACATGT A^C[GA][CT]GT
1631 ACACGT
Alw44I 1253 GTGCAC G^TGCAC
1810 GTGCAC
ApaLI 1253 GTGCAC G^TGCAC
1810 GTGCAC
AviII 254 TGCGCA TGC^GCA
1055 TGCGCA
BanI 825 GGTGCC G^G[CT][GA]CC
1303 GGCGCC
BbrPI 1275 CACGTG CAC^GTG
1893 CACGTG
BbsI 1071 GAAGAC GAAGAC(2/6)
1755 GAAGAC
BbvCI 1764 CCTCAGC CCTCAGC(-5/-2)
180 GCTGAGG
BfaI 383 CTAG C^TAG
986 CTAG
BglI 811 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1042 GCCAAGCTGGC
BpuAI 1071 GAAGAC GAAGAC(2/6)
1755 GAAGAC
BsaAI 1275 CACGTG [CT]AC^GT[GA]
1893 CACGTG
BspMI 160 GCAGGT ACCTGC(4/8)
1500 GCAGGT
BstUI 877 CGCG CG^CG
1549 CGCG
DraIII 263 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1486 CACCCCGTG
EarI 627 CTCTTC CTCTTC(1/4)
1296 GAAGAG
EcoNI 1167 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1431 CCTGCAGCAGG
FspI 254 TGCGCA TGC^GCA
1055 TGCGCA
Ksp632I 627 CTCTTC CTCTTC(1/4)
1296 GAAGAG
MaeI 383 CTAG C^TAG
986 CTAG
MvnI 877 CGCG CG^CG
1549 CGCG
NaeI 1370 GCCGGC GCC^GGC
1723 GCCGGC
NcoI 203 CCATGG C^CATGG
209 CCATGG
NgoMIV 1370 GCCGGC G^CCGGC
1723 GCCGGC
PaeR7I 336 CTCGAG C^TCGAG
1081 CTCGAG
PmaCI 1275 CACGTG CAC^GTG
1893 CACGTG
PmlI 1275 CACGTG CAC^GTG
1893 CACGTG
PpuMI 50 GGGACCC [GA]G^G[AT]CC[CT]
1777 GGGACCC
SacI 714 GAGCTC GAGCT^C
1818 GAGCTC
SfaNI 639 GATGC GCATC(5/9)
934 GCATC
XhoI 336 CTCGAG C^TCGAG
1081 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI