Restriction map for
pF1KE0326
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MaeI MamI MfeI MflI
MluI MroI MseI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 167 GGTACC G^GTACC
AccI 1474 GTCTAC GT^[AC][GT]AC
AlwI 276 GGATC GGATC(4/5)
ApaI 794 GGGCCC GGGCC^C
Asp718I 167 GGTACC G^GTACC
AspHI 832 GTGCTC G[AT]GC[AT]^C
BbrPI 467 CACGTG CAC^GTG
BsaAI 467 CACGTG [CT]AC^GT[GA]
BsaI 1324 GAGACC GGTCTC(1/5)
BsaWI 927 TCCGGT [AT]^CCGG[AT]
BsiHKAI 832 GTGCTC G[AT]GC[AT]^C
Bsp1407I 630 TGTACA T^GTACA
BsrDI 1317 CATTGC GCAATG(2/0)
BsrFI 1616 GCCGGC [GA]^CCGG[CT]
BsrGI 630 TGTACA T^GTACA
BssSI 682 CTCGTG CACGAG(-5/-1)
BstAPI 484 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1616 GCCGGC [GA]^CCGG[CT]
DraIII 485 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1436 GACTGGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1806 ATGCAT ATGCA^T
HincII 444 GTTGAC GT[CT]^[GA]AC
HindII 444 GTTGAC GT[CT]^[GA]AC
KpnI 167 GGTACC GGTAC^C
MaeII 468 ACGT A^CGT
MslI 678 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1616 GCCGGC GCC^GGC
NgoMIV 1616 GCCGGC G^CCGGC
NsiI 1806 ATGCAT ATGCA^T
NspI 1038 GCATGT [GA]CATG^[CT]
PmaCI 467 CACGTG CAC^GTG
PmlI 467 CACGTG CAC^GTG
PpuMI 977 AGGACCT [GA]G^G[AT]CC[CT]
SspBI 630 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 17 enzymes) #
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Asp700I 951 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1222 GAAATCCTTC
AvaI 520 CCCGAG C^[CT]CG[GA]G
1238 CTCGGG
BbsI 951 GAAGAC GAAGAC(2/6)
838 GTCTTC
BpuAI 951 GAAGAC GAAGAC(2/6)
838 GTCTTC
BseRI 886 CTCCTC GAGGAG(10/8)
439 GAGGAG
BsmAI 1324 GAGAC GTCTC(1/5)
1434 GAGAC
BsoBI 520 CCCGAG C^[CT]CG[GA]G
1238 CTCGGG
EarI 226 GAAGAG CTCTTC(1/4)
436 GAAGAG
EcoNI 474 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTCGACCAGG
Ksp632I 226 GAAGAG CTCTTC(1/4)
436 GAAGAG
MspA1I 280 CAGCTG C[AC]G^C[GT]G
1423 CAGCTG
NcoI 1109 CCATGG C^CATGG
1589 CCATGG
PleI 38 GACTC GAGTC(4/5)
1147 GACTC
PvuII 280 CAGCTG CAG^CTG
1423 CAGCTG
SmlI 712 CTCAAG C^T[CT][GA]AG
1383 CTTGAG
Tsp45I 905 GTGAC ^GT[GC]AC
1610 GTGAC
XmnI 951 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1222 GAAATCCTTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BglI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI TseI TspRI
TthHB8I XcmI