Restriction map for
pF1KB6803
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KpnI Ksp632I MaeI MamI
MfeI MluI MluNI MscI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 127 GTCTAC GT^[AC][GT]AC
AccIII 36 TCCGGA T^CCGGA
AluI 410 AGCT AG^CT
AlwNI 295 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 72 AGCGCT AGC^GCT
AvaI 472 CCCGAG C^[CT]CG[GA]G
AvaII 156 GGACC G^G[AT]CC
BamHI 393 GGATCC G^GATCC
BbeI 316 GGCGCC GGCGC^C
BlpI 411 GCTCAGC GC^T[ACGT]AGC
BmyI 454 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 411 GCTCAGC GC^T[ACGT]AGC
BsaWI 36 TCCGGA [AT]^CCGG[AT]
BseAI 36 TCCGGA T^CCGGA
BsoBI 472 CCCGAG C^[CT]CG[GA]G
Bsp1286I 454 GTGCCC G[AGT]GC[ACT]^C
BspEI 36 TCCGGA T^CCGGA
BspMI 347 ACCTGC ACCTGC(4/8)
BsrI 449 ACTGG ACTGG(1/-1)
BstAPI 78 GCATGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 366 TTCGAA TT^CGAA
CelII 411 GCTCAGC GC^T[ACGT]AGC
Cfr13I 156 GGACC G^G[ACGT]CC
DdeI 412 CTCAG C^T[ACGT]AG
DsaI 18 CCGCGG C^C[GA][CT]GG
EaeI 21 CGGCCA [CT]^GGCC[GA]
Eco47III 72 AGCGCT AGC^GCT
EcoT14I 401 CCTTGG C^C[AT][AT]GG
HaeIII 22 GGCC GG^CC
HincII 442 GTTAAC GT[CT]^[GA]AC
HindII 442 GTTAAC GT[CT]^[GA]AC
HpaI 442 GTTAAC GTT^AAC
KasI 316 GGCGCC G^GCGCC
KspI 18 CCGCGG CCGC^GG
MaeII 152 ACGT A^CGT
MaeIII 169 GTGAC ^GT[ACGT]AC
MboII 10 GAAGA GAAGA(8/7)
MroI 36 TCCGGA T^CCGGA
MseI 443 TTAA T^TAA
NarI 316 GGCGCC GG^CGCC
NlaIII 79 CATG CATG^
NspV 366 TTCGAA TT^CGAA
PstI 220 CTGCAG CTGCA^G
SacII 18 CCGCGG CCGC^GG
Sau96I 156 GGACC G^G[ACGT]CC
SfcI 220 CTGCAG C^T[GA][CT]AG
SfoI 316 GGCGCC GGC^GCC
SfuI 366 TTCGAA TT^CGAA
SgrAI 318 CGCCGGCG C[GA]^CCGG[CT]G
StyI 401 CCTTGG C^C[AT][AT]GG
Tru9I 443 TTAA T^TAA
Tsp45I 169 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BcnI 65 CCGGG CC^[GC]GG
340 CCGGG
BsaJI 18 CCGCGG C^C[ACGT][ACGT]GG
401 CCTTGG
BsgI 349 CTGCAC GTGCAG(16/14)
292 GTGCAG
BsiYI 18 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCCCAACGAGG
BslI 18 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCCCAACGAGG
BsrFI 121 GCCGGC [GA]^CCGG[CT]
319 GCCGGC
BstF5I 357 CATCC GGATG(2/0)
53 GGATG
BstYI 224 AGATCC [GA]^GATC[CT]
393 GGATCC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
319 GCCGGC
FokI 357 CATCC GGATG(9/13)
53 GGATG
HaeII 72 AGCGCT [GA]GCGC^[CT]
316 GGCGCC
HinfI 3 GACTC G^A[ACGT]TC
133 GAGTC
HphI 193 GGTGA GGTGA(8/7)
464 TCACC
MflI 224 AGATCC [GA]^GATC[CT]
393 GGATCC
MspA1I 18 CCGCGG C[AC]G^C[GT]G
295 CAGCGG
NaeI 121 GCCGGC GCC^GGC
319 GCCGGC
NciI 65 CCGGG CC^[GC]GG
340 CCGGG
NgoMIV 121 GCCGGC G^CCGGC
319 GCCGGC
PleI 3 GACTC GAGTC(4/5)
133 GAGTC
TaqI 269 TCGA T^CGA
367 TCGA
TspRI 117 CACTG CA[GC]TG[ACGT][ACGT]^
352 CACTG
TthHB8I 269 TCGA T^CGA
367 TCGA
XhoII 224 AGATCC [GA]^GATC[CT]
393 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AcyI AlwI BanI
BbvI BsaHI BseRI BssKI BstNI
BstUI Cac8I CfoI DpnII EcoRII
FauI Fnu4HI HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MboI
MnlI MspI MvaI MvnI MwoI
NdeII NlaIV Sau3AI ScrFI SfaNI
TseI