Restriction map for
pF1KE0108
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# NO CUT ENZYMES ( 154 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiHKAI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 13 GGTACC G^GTACC
AgeI 5 ACCGGT A^CCGGT
Asp718I 13 GGTACC G^GTACC
BamHI 887 GGATCC G^GATCC
BbsI 164 GAAGAC GAAGAC(2/6)
BcnI 17 CCCGG CC^[GC]GG
BlpI 486 GCTAAGC GC^T[ACGT]AGC
BmyI 185 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 486 GCTAAGC GC^T[ACGT]AGC
BpuAI 164 GAAGAC GAAGAC(2/6)
BsaI 474 GAGACC GGTCTC(1/5)
BsaWI 5 ACCGGT [AT]^CCGG[AT]
BseRI 169 CTCCTC GAGGAG(10/8)
BsgI 595 GTGCAG GTGCAG(16/14)
BsiYI 18 CCGGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 18 CCGGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 285 GAGACG CGTCTC(1/5)
Bsp1286I 185 GTGCCC G[AGT]GC[ACT]^C
BsrBI 877 GAGCGG CCGCTC(-3/-3)
BsrFI 5 ACCGGT [GA]^CCGG[CT]
BstBI 338 TTCGAA TT^CGAA
BstXI 174 CCAGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 887 GGATCC [GA]^GATC[CT]
CelII 486 GCTAAGC GC^T[ACGT]AGC
Cfr10I 5 ACCGGT [GA]^CCGG[CT]
DpnII 888 GATC ^GATC
EcoRV 106 GATATC GAT^ATC
HphI 944 TCACC GGTGA(8/7)
KpnI 13 GGTACC GGTAC^C
MboI 888 GATC ^GATC
MflI 887 GGATCC [GA]^GATC[CT]
MwoI 482 GCCAGCTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 17 CCCGG CC^[GC]GG
NdeII 888 GATC ^GATC
NspV 338 TTCGAA TT^CGAA
PinAI 5 ACCGGT A^CCGGT
PleI 167 GACTC GAGTC(4/5)
PstI 826 CTGCAG CTGCA^G
Sau3AI 888 GATC ^GATC
SfcI 826 CTGCAG C^T[GA][CT]AG
SfuI 338 TTCGAA TT^CGAA
SmlI 469 CTCAAG C^T[CT][GA]AG
SpeI 247 ACTAGT A^CTAGT
SspI 395 AATATT AAT^ATT
StuI 585 AGGCCT AGG^CCT
XcmI 222 CCAACAGAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 887 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AatII 287 GACGTC GACGT^C
796 GACGTC
AcyI 287 GACGTC G[GA]^CG[CT]C
796 GACGTC
AlwI 888 GATCC GGATC(4/5)
887 GGATC
BanI 13 GGTACC G^G[CT][GA]CC
184 GGTGCC
BbvI 65 GCAGC GCAGC(8/12)
316 GCAGC
BpmI 172 CTCCAG CTGGAG(16/14)
710 CTGGAG
BsaHI 287 GACGTC G[GA]^CG[CT]C
796 GACGTC
BsaJI 32 CCACGG C^C[ACGT][ACGT]GG
947 CCGTGG
BsrDI 68 GCAATG GCAATG(2/0)
720 GCAATG
Cfr13I 60 GGCCC G^G[ACGT]CC
600 GGCCC
DsaI 32 CCACGG C^C[GA][CT]GG
947 CCGTGG
EarI 266 GAAGAG CTCTTC(1/4)
581 GAAGAG
EcoRI 228 GAATTC G^AATTC
335 GAATTC
FauI 62 CCCGC CCCGC(4/6)
783 GCGGG
HapII 6 CCGG C^CGG
18 CCGG
Hin1I 287 GACGTC G[GA]^CG[CT]C
796 GACGTC
HpaII 6 CCGG C^CGG
18 CCGG
Ksp632I 266 GAAGAG CTCTTC(1/4)
581 GAAGAG
MspI 6 CCGG C^CGG
18 CCGG
Sau96I 60 GGCCC G^G[ACGT]CC
600 GGCCC
TseI 65 GCAGC G^C[AT]GC
316 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BfaI BsmAI BsrI BssKI BstF5I
BstNI Cac8I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeII MaeIII MboII MnlI MseI
MvaI NlaIV RsaI ScrFI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I