Restriction map for
pF1KB6838
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 133 GCGG CCGC(-3/-1)
AfaI 90 GTAC GT^AC
AlwI 452 GGATC GGATC(4/5)
AspHI 409 GAGCTC G[AT]GC[AT]^C
BanII 409 GAGCTC G[GA]GC[CT]^C
BbsI 54 GAAGAC GAAGAC(2/6)
BbvI 123 GCAGC GCAGC(8/12)
BglII 149 AGATCT A^GATCT
BmyI 409 GAGCTC G[AGT]GC[ACT]^C
BpuAI 54 GAAGAC GAAGAC(2/6)
BsaI 176 GAGACC GGTCTC(1/5)
BsaWI 449 ACCGGA [AT]^CCGG[AT]
BsgI 538 GTGCAG GTGCAG(16/14)
BsiHKAI 409 GAGCTC G[AT]GC[AT]^C
BsmAI 176 GAGAC GTCTC(1/5)
BsmFI 549 GTCCC GGGAC(10/14)
BsmI 474 GAATGC GAATGC(1/-1)
Bsp1286I 409 GAGCTC G[AGT]GC[ACT]^C
BsrI 116 CCAGT ACTGG(1/-1)
BstEII 270 GGTGACC G^GT[ACGT]ACC
BstPI 270 GGTGACC G^GT[ACGT]ACC
DdeI 434 CTGAG C^T[ACGT]AG
DsaI 404 CCGTGG C^C[GA][CT]GG
EarI 438 GAAGAG CTCTTC(1/4)
EcoO65I 270 GGTGACC G^GT[ACGT]ACC
EcoT14I 518 CCAAGG C^C[AT][AT]GG
HaeIII 309 GGCC GG^CC
HapII 450 CCGG C^CGG
HpaII 450 CCGG C^CGG
Ksp632I 438 GAAGAG CTCTTC(1/4)
MaeII 209 ACGT A^CGT
MaeIII 271 GTGAC ^GT[ACGT]AC
MspA1I 124 CAGCTG C[AC]G^C[GT]G
MspI 450 CCGG C^CGG
MwoI 489 GCTGACCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 462 CATG CATG^
PflMI 387 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 496 CTGCAG CTGCA^G
PvuII 124 CAGCTG CAG^CTG
RsaI 90 GTAC GT^AC
SacI 409 GAGCTC GAGCT^C
SbfI 495 CCTGCAGG CCTGCA^GG
SfcI 496 CTGCAG C^T[GA][CT]AG
Sse8387I 495 CCTGCAGG CCTGCA^GG
StyI 518 CCAAGG C^C[AT][AT]GG
TseI 123 GCAGC G^C[AT]GC
Tsp45I 271 GTGAC ^GT[GC]AC
Van91I 387 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 259 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
541 CAGGCGCTG
AvaII 78 GGTCC G^G[AT]CC
447 GGACC
BsiYI 387 CCAGATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCCAGAGGAGG
BslI 387 CCAGATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCCAGAGGAGG
BstYI 149 AGATCT [GA]^GATC[CT]
452 GGATCT
Cac8I 136 GCAGGC GC[ACGT]^[ACGT]GC
540 GCAGGC
CfoI 262 GCGC GCG^C
544 GCGC
DpnII 150 GATC ^GATC
453 GATC
Fnu4HI 123 GCAGC GC^[ACGT]GC
133 GCGGC
HaeII 261 GGCGCT [GA]GCGC^[CT]
543 GGCGCT
HhaI 262 GCGC GCG^C
544 GCGC
HinP1I 262 GCGC G^CGC
544 GCGC
HphI 270 GGTGA GGTGA(8/7)
347 TCACC
ItaI 123 GCAGC GC^[ACGT]GC
133 GCGGC
MboI 150 GATC ^GATC
453 GATC
MflI 149 AGATCT [GA]^GATC[CT]
452 GGATCT
NdeII 150 GATC ^GATC
453 GATC
NlaIV 283 GGAACC GG[ACGT]^[ACGT]CC
514 GGTTCC
Sau3AI 150 GATC ^GATC
453 GATC
Tsp509I 161 AATT ^AATT
237 AATT
TspRI 85 CAGTG CA[GC]TG[ACGT][ACGT]^
117 CAGTG
XcmI 116 CCAGTGGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
380 CCATTTACCAGATGG
XhoII 149 AGATCT [GA]^GATC[CT]
452 GGATCT
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BsaJI BseRI BssKI BstF5I
BstNI Cfr13I EcoRII FokI MboII
MnlI MvaI Sau96I ScrFI SfaNI