Restriction map for
pF1KB6365
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CelII
ClaI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 418 AAATTT [GA]^AATT[CT]
AcyI 254 GGCGCC G[GA]^CG[CT]C
AlwNI 48 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 154 AGCGCT AGC^GCT
ApoI 418 AAATTT [GA]^AATT[CT]
BamHI 331 GGATCC G^GATCC
BanI 254 GGCGCC G^G[CT][GA]CC
BbeI 254 GGCGCC GGCGC^C
BbsI 162 GTCTTC GAAGAC(2/6)
BglI 257 GCCGGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 305 AGATCT A^GATCT
BpmI 129 CTCCAG CTGGAG(16/14)
BpuAI 162 GTCTTC GAAGAC(2/6)
BsaHI 254 GGCGCC G[GA]^CG[CT]C
BsmBI 73 CGTCTC CGTCTC(1/5)
BsrFI 262 ACCGGC [GA]^CCGG[CT]
BsrI 237 CCAGT ACTGG(1/-1)
BstF5I 315 GGATG GGATG(2/0)
BstNI 53 CCTGG CC^[AT]GG
BstXI 207 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 262 ACCGGC [GA]^CCGG[CT]
CpoI 259 CGGACCG CG^G[AT]CCG
Eco47III 154 AGCGCT AGC^GCT
EcoRII 53 CCTGG ^CC[AT]GG
EcoT14I 207 CCAAGG C^C[AT][AT]GG
FokI 315 GGATG GGATG(9/13)
HaeIII 570 GGCC GG^CC
HgaI 72 GCGTC GACGC(5/10)
Hin1I 254 GGCGCC G[GA]^CG[CT]C
KasI 254 GGCGCC G^GCGCC
MaeIII 691 GTTAC ^GT[ACGT]AC
MslI 451 CATGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 101 CCGCTG C[AC]G^C[GT]G
MvaI 53 CCTGG CC^[AT]GG
NarI 254 GGCGCC GG^CGCC
NlaIII 451 CATG CATG^
PleI 785 GACTC GAGTC(4/5)
RsrII 259 CGGACCG CG^G[AT]CCG
SfaNI 287 GCATC GCATC(5/9)
SfoI 254 GGCGCC GGC^GCC
SmaI 611 CCCGGG CCC^GGG
SmlI 787 CTCAAG C^T[CT][GA]AG
SspI 366 AATATT AAT^ATT
StyI 207 CCAAGG C^C[AT][AT]GG
Tsp509I 419 AATT ^AATT
XmaCI 611 CCCGGG C^CCGGG
XmaI 611 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 249 CTCGGG C^[CT]CG[GA]G
611 CCCGGG
AvaII 97 GGACC G^G[AT]CC
260 GGACC
BanII 56 GGGCTC G[GA]GC[CT]^C
243 GAGCCC
BbvCI 45 CCTCAGC CCTCAGC(-5/-2)
524 CCTCAGC
BfaI 122 CTAG C^TAG
719 CTAG
BmyI 56 GGGCTC G[AGT]GC[ACT]^C
243 GAGCCC
BsgI 749 CTGCAC GTGCAG(16/14)
603 GTGCAG
BsmAI 74 GTCTC GTCTC(1/5)
378 GTCTC
BsoBI 249 CTCGGG C^[CT]CG[GA]G
611 CCCGGG
Bsp1286I 56 GGGCTC G[AGT]GC[ACT]^C
243 GAGCCC
BstYI 305 AGATCT [GA]^GATC[CT]
331 GGATCC
EarI 25 CTCTTC CTCTTC(1/4)
168 CTCTTC
Ksp632I 25 CTCTTC CTCTTC(1/4)
168 CTCTTC
MaeI 122 CTAG C^TAG
719 CTAG
MflI 305 AGATCT [GA]^GATC[CT]
331 GGATCC
MseI 352 TTAA T^TAA
617 TTAA
PstI 276 CTGCAG CTGCA^G
301 CTGCAG
SfcI 276 CTGCAG C^T[GA][CT]AG
301 CTGCAG
TfiI 653 GATTC G^A[AT]TC
738 GATTC
Tru9I 352 TTAA T^TAA
617 TTAA
XhoII 305 AGATCT [GA]^GATC[CT]
331 GGATCC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BsaJI BseRI BsiYI BslI BssKI
CfoI Cfr13I DdeI DpnII FauI
Fnu4HI HaeII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MnlI MspI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI