Restriction map for
pF1KE0043
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfaNI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 512 CGCG CG^CG
AciI 708 GCGG CCGC(-3/-1)
AflII 993 CTTAAG C^TTAAG
AflIII 128 ACGTGT A^C[GA][CT]GT
AgeI 814 ACCGGT A^CCGGT
Alw44I 1506 GTGCAC G^TGCAC
AocI 318 CCTCAGG CC^T[ACGT]AGG
ApaLI 1506 GTGCAC G^TGCAC
AseI 1572 ATTAAT AT^TAAT
AsnI 1572 ATTAAT AT^TAAT
AspHI 1506 GTGCAC G[AT]GC[AT]^C
AvaI 252 CCCGGG C^[CT]CG[GA]G
BanII 308 GAGCCC G[GA]GC[CT]^C
BbvCI 603 GCTGAGG CCTCAGC(-5/-2)
BclI 1604 TGATCA T^GATCA
BfrI 993 CTTAAG C^TTAAG
BsaWI 814 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1506 GTGCAC G[AT]GC[AT]^C
BsoBI 252 CCCGGG C^[CT]CG[GA]G
BsrFI 814 ACCGGT [GA]^CCGG[CT]
BssSI 583 CTCGTG CACGAG(-5/-1)
BstUI 512 CGCG CG^CG
BstXI 293 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 318 CCTCAGG CC^T[ACGT]AGG
CfoI 116 GCGC GCG^C
Cfr10I 814 ACCGGT [GA]^CCGG[CT]
DraIII 1154 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 293 CCATGG C^C[GA][CT]GG
Eco81I 318 CCTCAGG CC^T[ACGT]AGG
FauI 708 GCGGG CCCGC(4/6)
FbaI 1604 TGATCA T^GATCA
HaeII 115 AGCGCC [GA]GCGC^[CT]
HgaI 54 GACGC GACGC(5/10)
HhaI 116 GCGC GCG^C
HinP1I 116 GCGC G^CGC
HincII 553 GTCAAC GT[CT]^[GA]AC
HindII 553 GTCAAC GT[CT]^[GA]AC
MvnI 512 CGCG CG^CG
MwoI 647 GCCTGTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 293 CCATGG C^CATGG
PflMI 426 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 814 ACCGGT A^CCGGT
PpuMI 248 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1572 ATTAAT AT^TAAT
PstI 215 CTGCAG CTGCA^G
SapI 1471 GAAGAGC GCTCTTC(1/4)
SfcI 215 CTGCAG C^T[GA][CT]AG
SgrAI 813 CACCGGTG C[GA]^CCGG[CT]G
SmaI 252 CCCGGG CCC^GGG
SmlI 993 CTTAAG C^T[CT][GA]AG
TfiI 1162 GATTC G^A[AT]TC
Van91I 426 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 489 CCAGATCCGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 252 CCCGGG C^CCGGG
XmaI 252 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 4 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAAGAGCTTC
AspI 244 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1339 GACAAAGTC
BbvI 211 GCAGC GCAGC(8/12)
214 GCTGC
BglII 62 AGATCT A^GATCT
1557 AGATCT
BpmI 58 CTGGAG CTGGAG(16/14)
1543 CTGGAG
Cac8I 1084 GCAGGC GC[ACGT]^[ACGT]GC
1372 GCTGGC
DraII 248 AGGTCCC [GA]G^G[ACGT]CC[CT]
1313 AGGCCCT
EcoO109I 248 AGGTCCC [GA]G^G[ACGT]CC[CT]
1313 AGGCCCT
EcoRI 371 GAATTC G^AATTC
1435 GAATTC
EcoT14I 293 CCATGG C^C[AT][AT]GG
570 CCAAGG
Fnu4HI 211 GCAGC GC^[ACGT]GC
214 GCTGC
ItaI 211 GCAGC GC^[ACGT]GC
214 GCTGC
MaeII 128 ACGT A^CGT
551 ACGT
MslI 294 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
357 CATTCTTGTG
MspA1I 212 CAGCTG C[AC]G^C[GT]G
654 CAGCTG
PflFI 244 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1339 GACAAAGTC
PleI 515 GAGTC GAGTC(4/5)
712 GAGTC
PvuII 212 CAGCTG CAG^CTG
654 CAGCTG
SspI 1051 AATATT AAT^ATT
1575 AATATT
StyI 293 CCATGG C^C[AT][AT]GG
570 CCAAGG
TseI 211 GCAGC G^C[AT]GC
214 GCTGC
Tth111I 244 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1339 GACAAAGTC
XmnI 4 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbsI BcnI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII FokI HaeIII
HapII HindIII HinfI HpaII HphI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SexAI TaqI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XhoII