Restriction map for
pF1KB6350
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 58 CGCG CG^CG
AfaI 771 GTAC GT^AC
AspHI 628 GAGCAC G[AT]GC[AT]^C
BbsI 546 GAAGAC GAAGAC(2/6)
BbvI 324 GCTGC GCAGC(8/12)
BciVI 90 GGATAC GTATCC(6/5)
BglII 367 AGATCT A^GATCT
BmyI 628 GAGCAC G[AGT]GC[ACT]^C
BpuAI 546 GAAGAC GAAGAC(2/6)
BsaI 295 GAGACC GGTCTC(1/5)
BsiHKAI 628 GAGCAC G[AT]GC[AT]^C
Bsp1286I 628 GAGCAC G[AGT]GC[ACT]^C
BspHI 558 TCATGA T^CATGA
BssSI 471 CACGAG CACGAG(-5/-1)
BstAPI 160 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 58 CGCG CG^CG
BstYI 367 AGATCT [GA]^GATC[CT]
DraII 413 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 451 GACGGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 126 CCATGG C^C[GA][CT]GG
EcoO109I 413 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 699 GATATC GAT^ATC
EcoT14I 126 CCATGG C^C[AT][AT]GG
FauI 120 GCGGG CCCGC(4/6)
HindIII 209 AAGCTT A^AGCTT
MaeII 133 ACGT A^CGT
MflI 367 AGATCT [GA]^GATC[CT]
MseI 814 TTAA T^TAA
MvnI 58 CGCG CG^CG
MwoI 160 GCATCTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 126 CCATGG C^CATGG
PpuMI 413 GGGACCT [GA]G^G[AT]CC[CT]
PstI 371 CTGCAG CTGCA^G
RcaI 558 TCATGA T^CATGA
RsaI 771 GTAC GT^AC
SfcI 371 CTGCAG C^T[GA][CT]AG
StyI 126 CCATGG C^C[AT][AT]GG
TaqI 690 TCGA T^CGA
Tru9I 814 TTAA T^TAA
TseI 324 GCTGC G^C[AT]GC
TthHB8I 690 TCGA T^CGA
XcmI 299 CCAACTCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 367 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 57 CCGC CCGC(-3/-1)
120 GCGG
BanI 81 GGTGCC G^G[CT][GA]CC
574 GGTGCC
BbvCI 357 GCTGAGG CCTCAGC(-5/-2)
427 GCTGAGG
BcnI 85 CCGGG CC^[GC]GG
704 CCCGG
BfaI 76 CTAG C^TAG
496 CTAG
BsmFI 255 GGGAC GGGAC(10/14)
413 GGGAC
BsrI 51 ACTGG ACTGG(1/-1)
22 CCAGT
Fnu4HI 56 GCCGC GC^[ACGT]GC
324 GCTGC
HaeIII 55 GGCC GG^CC
124 GGCC
HapII 85 CCGG C^CGG
705 CCGG
HpaII 85 CCGG C^CGG
705 CCGG
ItaI 56 GCCGC GC^[ACGT]GC
324 GCTGC
MaeI 76 CTAG C^TAG
496 CTAG
MaeIII 593 GTAAC ^GT[ACGT]AC
733 GTAAC
MslI 246 CATCGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
553 CACTTTCATG
MspI 85 CCGG C^CGG
705 CCGG
NciI 85 CCGGG CC^[GC]GG
704 CCCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AvaII BpmI BsaJI BseRI
BsiYI BslI BsmAI BsrDI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FokI HinfI
HphI Ksp632I MboI MboII MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI TspRI