Restriction map for
pF1KB5951
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BfaI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TspRI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1330 GTCTAC GT^[AC][GT]AC
AcsI 1336 AAATTT [GA]^AATT[CT]
ApoI 1336 AAATTT [GA]^AATT[CT]
BbsI 1459 GAAGAC GAAGAC(2/6)
BciVI 633 GTATCC GTATCC(6/5)
BclI 15 TGATCA T^GATCA
BglII 111 AGATCT A^GATCT
BlpI 1263 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1263 GCTGAGC GC^T[ACGT]AGC
BpuAI 1459 GAAGAC GAAGAC(2/6)
BsaWI 1200 ACCGGA [AT]^CCGG[AT]
BsgI 274 CTGCAC GTGCAG(16/14)
BsmI 61 GAATGC GAATGC(1/-1)
BsrBI 1269 CCGCTC CCGCTC(-3/-3)
BsrDI 1158 CATTGC GCAATG(2/0)
BsrFI 1238 ACCGGC [GA]^CCGG[CT]
BstEII 1122 GGTCACC G^GT[ACGT]ACC
BstPI 1122 GGTCACC G^GT[ACGT]ACC
CelII 1263 GCTGAGC GC^T[ACGT]AGC
CfoI 1218 GCGC GCG^C
Cfr10I 1238 ACCGGC [GA]^CCGG[CT]
DsaI 1367 CCATGG C^C[GA][CT]GG
EaeI 1240 CGGCCA [CT]^GGCC[GA]
EcoO65I 1122 GGTCACC G^GT[ACGT]ACC
EcoT14I 1367 CCATGG C^C[AT][AT]GG
FbaI 15 TGATCA T^GATCA
HaeII 1217 GGCGCT [GA]GCGC^[CT]
HhaI 1218 GCGC GCG^C
HinP1I 1218 GCGC G^CGC
HincII 152 GTCAAC GT[CT]^[GA]AC
HindII 152 GTCAAC GT[CT]^[GA]AC
MaeII 1328 ACGT A^CGT
MseI 400 TTAA T^TAA
NcoI 1367 CCATGG C^CATGG
NdeI 977 CATATG CA^TATG
PflMI 544 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1408 GAGTC GAGTC(4/5)
PpuMI 821 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 659 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 130 GAAGAGC GCTCTTC(1/4)
SexAI 230 ACCTGGT A^CC[AT]GGT
SmaI 825 CCCGGG CCC^GGG
StyI 1367 CCATGG C^C[AT][AT]GG
Tru9I 400 TTAA T^TAA
Tsp509I 1337 AATT ^AATT
Van91I 544 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 825 CCCGGG C^CCGGG
XmaI 825 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 988 GATCC GGATC(4/5)
144 GGATC
AvaI 825 CCCGGG C^[CT]CG[GA]G
887 CTCGGG
BbvCI 691 CCTCAGC CCTCAGC(-5/-2)
1422 CCTCAGC
BcgI 1515 CGAAGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
879 GCAGGAGCCTCG
BsmAI 657 GAGAC GTCTC(1/5)
1029 GAGAC
BsoBI 825 CCCGGG C^[CT]CG[GA]G
887 CTCGGG
BstF5I 929 CATCC GGATG(2/0)
1227 CATCC
FokI 929 CATCC GGATG(9/13)
1227 CATCC
HgaI 899 GCGTC GACGC(5/10)
1389 GCGTC
NspI 483 GCATGC [GA]CATG^[CT]
830 GCATGC
PvuII 494 CAGCTG CAG^CTG
1105 CAGCTG
SphI 483 GCATGC GCATG^C
830 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwNI AocI ApaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoNI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I TthHB8I
XhoII