Restriction map for
pF1KB6552
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 319 GTCTAC GT^[AC][GT]AC
AcsI 466 GAATTC [GA]^AATT[CT]
AcyI 652 GACGCC G[GA]^CG[CT]C
ApoI 466 GAATTC [GA]^AATT[CT]
AvaI 1038 CTCGGG C^[CT]CG[GA]G
AvrII 183 CCTAGG C^CTAGG
BalI 1004 TGGCCA TGG^CCA
BanI 103 GGCACC G^G[CT][GA]CC
BbsI 1093 GTCTTC GAAGAC(2/6)
BglI 839 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 183 CCTAGG C^CTAGG
BpuAI 1093 GTCTTC GAAGAC(2/6)
BsaHI 652 GACGCC G[GA]^CG[CT]C
BsaI 1107 GGTCTC GGTCTC(1/5)
BsaWI 1104 TCCGGT [AT]^CCGG[AT]
BsiEI 856 CGGTCG CG[GA][CT]^CG
BsoBI 1038 CTCGGG C^[CT]CG[GA]G
BspDI 796 ATCGAT AT^CGAT
BspMI 1148 ACCTGC ACCTGC(4/8)
BssHII 339 GCGCGC G^CGCGC
ClaI 796 ATCGAT AT^CGAT
DdeI 230 CTAAG C^T[ACGT]AG
EcoNI 351 CCTCCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 466 GAATTC G^AATTC
HgaI 652 GACGC GACGC(5/10)
Hin1I 652 GACGCC G[GA]^CG[CT]C
HincII 193 GTCAAC GT[CT]^[GA]AC
HindII 193 GTCAAC GT[CT]^[GA]AC
HinfI 991 GAGTC G^A[ACGT]TC
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
MseI 929 TTAA T^TAA
NheI 1030 GCTAGC G^CTAGC
NspI 644 ACATGC [GA]CATG^[CT]
PflMI 996 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 991 GAGTC GAGTC(4/5)
StuI 914 AGGCCT AGG^CCT
Tru9I 929 TTAA T^TAA
Tsp45I 4 GTGAC ^GT[GC]AC
Tsp509I 467 AATT ^AATT
Van91I 996 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 832 GTGCAC G^TGCAC
1120 GTGCAC
AlwI 255 GATCC GGATC(4/5)
594 GATCC
ApaLI 832 GTGCAC G^TGCAC
1120 GTGCAC
BanII 234 GGGCTC G[GA]GC[CT]^C
529 GAGCCC
BfaI 184 CTAG C^TAG
1031 CTAG
BpmI 247 CTGGAG CTGGAG(16/14)
907 CTGGAG
BsgI 1150 CTGCAC GTGCAG(16/14)
382 GTGCAG
BsrBI 120 GAGCGG CCGCTC(-3/-3)
1054 GAGCGG
BsrI 518 CCAGT ACTGG(1/-1)
736 CCAGT
BstXI 482 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCATCTGCCTGG
BstYI 254 AGATCC [GA]^GATC[CT]
593 AGATCC
DpnII 255 GATC ^GATC
594 GATC
EaeI 860 CGGCCA [CT]^GGCC[GA]
1004 TGGCCA
HaeII 387 GGCGCT [GA]GCGC^[CT]
935 AGCGCC
MaeI 184 CTAG C^TAG
1031 CTAG
MaeII 197 ACGT A^CGT
1009 ACGT
MaeIII 4 GTGAC ^GT[ACGT]AC
206 GTAAC
MboI 255 GATC ^GATC
594 GATC
MflI 254 AGATCC [GA]^GATC[CT]
593 AGATCC
MslI 210 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
732 CATCCCAGTG
NdeII 255 GATC ^GATC
594 GATC
PvuII 976 CAGCTG CAG^CTG
1176 CAGCTG
Sau3AI 255 GATC ^GATC
594 GATC
XcmI 524 CCACAGAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
863 CCACCATCTGCCTGG
XhoII 254 AGATCC [GA]^GATC[CT]
593 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PpuMI PstI RsaI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I