Restriction map for
pF1KB6533
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI HphI KpnI Ksp632I
MamI MflI MluI MluNI MroI
MscI MseI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SapI SbfI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccI 417 GTCGAC GT^[AC][GT]AC
BbsI 352 GTCTTC GAAGAC(2/6)
BciVI 370 GGATAC GTATCC(6/5)
BclI 608 TGATCA T^GATCA
BfaI 452 CTAG C^TAG
BpuAI 352 GTCTTC GAAGAC(2/6)
BsmI 744 GAATGC GAATGC(1/-1)
BsrDI 66 GCAATG GCAATG(2/0)
BsrI 654 CCAGT ACTGG(1/-1)
BstXI 176 CCATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 608 TGATCA T^GATCA
KspI 213 CCGCGG CCGC^GG
MaeI 452 CTAG C^TAG
MaeIII 647 GTCAC ^GT[ACGT]AC
MfeI 624 CAATTG C^AATTG
MunI 624 CAATTG C^AATTG
NotI 215 GCGGCCGC GC^GGCCGC
NspI 578 ACATGC [GA]CATG^[CT]
SacI 208 GAGCTC GAGCT^C
SacII 213 CCGCGG CCGC^GG
SalI 417 GTCGAC G^TCGAC
ScaI 448 AGTACT AGT^ACT
SfiI 306 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 472 GAATC G^A[AT]TC
Tsp45I 647 GTCAC ^GT[GC]AC
Tsp509I 625 AATT ^AATT
XcmI 167 CCAAGGGCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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BglI 307 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
773 GCCAGGCAGGC
BlpI 432 GCTCAGC GC^T[ACGT]AGC
1059 GCTGAGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
1059 GCTGAGC
BsgI 405 GTGCAG GTGCAG(16/14)
480 GTGCAG
BsmAI 616 GTCTC GTCTC(1/5)
1027 GTCTC
BsmBI 615 CGTCTC CGTCTC(1/5)
1026 CGTCTC
BsrBI 87 CCGCTC CCGCTC(-3/-3)
59 GAGCGG
CelII 432 GCTCAGC GC^T[ACGT]AGC
1059 GCTGAGC
DpnII 508 GATC ^GATC
609 GATC
DraIII 190 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
241 CACGCCGTG
EagI 216 CGGCCG C^GGCCG
314 CGGCCG
EclXI 216 CGGCCG C^GGCCG
314 CGGCCG
Eco52I 216 CGGCCG C^GGCCG
314 CGGCCG
HgaI 25 GACGC GACGC(5/10)
455 GCGTC
HincII 130 GTCAAC GT[CT]^[GA]AC
417 GTCGAC
HindII 130 GTCAAC GT[CT]^[GA]AC
417 GTCGAC
MaeII 134 ACGT A^CGT
614 ACGT
MboI 508 GATC ^GATC
609 GATC
NdeII 508 GATC ^GATC
609 GATC
PflMI 240 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCAGGTGCTGG
PstI 489 CTGCAG CTGCA^G
916 CTGCAG
PvuII 910 CAGCTG CAG^CTG
1095 CAGCTG
Sau3AI 508 GATC ^GATC
609 GATC
SfcI 489 CTGCAG C^T[GA][CT]AG
916 CTGCAG
StuI 33 AGGCCT AGG^CCT
848 AGGCCT
Van91I 240 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrFI BssHII BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI SfoI StyI TaqI TseI
TspRI TthHB8I