Restriction map for
pF1KB6446
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI DsaI Eco47III
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HinfI HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 974 GGTACC G^GTACC
AccII 355 CGCG CG^CG
AcyI 670 GACGCC G[GA]^CG[CT]C
AhdI 905 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 510 GATCC GGATC(4/5)
AocI 817 CCTGAGG CC^T[ACGT]AGG
Asp718I 974 GGTACC G^GTACC
AspEI 905 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 57 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 727 GTCTTC GAAGAC(2/6)
BciVI 777 GTATCC GTATCC(6/5)
BfaI 229 CTAG C^TAG
BpmI 178 CTCCAG CTGGAG(16/14)
BpuAI 727 GTCTTC GAAGAC(2/6)
BsaHI 670 GACGCC G[GA]^CG[CT]C
BsgI 948 CTGCAC GTGCAG(16/14)
BsiEI 606 CGGCCG CG[GA][CT]^CG
BsmBI 865 CGTCTC CGTCTC(1/5)
BsrFI 539 GCCGGC [GA]^CCGG[CT]
BsrI 720 CCAGT ACTGG(1/-1)
BstUI 355 CGCG CG^CG
BstYI 509 AGATCC [GA]^GATC[CT]
Bsu36I 817 CCTGAGG CC^T[ACGT]AGG
CfoI 356 GCGC GCG^C
Cfr10I 539 GCCGGC [GA]^CCGG[CT]
DpnII 510 GATC ^GATC
DraIII 130 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 606 CGGCCG [CT]^GGCC[GA]
EagI 606 CGGCCG C^GGCCG
Eam1105I 905 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 606 CGGCCG C^GGCCG
Eco52I 606 CGGCCG C^GGCCG
Eco81I 817 CCTGAGG CC^T[ACGT]AGG
EcoNI 456 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 732 CCCGC CCCGC(4/6)
HhaI 356 GCGC GCG^C
Hin1I 670 GACGCC G[GA]^CG[CT]C
HinP1I 356 GCGC G^CGC
KpnI 974 GGTACC GGTAC^C
MaeI 229 CTAG C^TAG
MaeIII 55 GTGAC ^GT[ACGT]AC
MboI 510 GATC ^GATC
MflI 509 AGATCC [GA]^GATC[CT]
MvnI 355 CGCG CG^CG
NaeI 539 GCCGGC GCC^GGC
NdeII 510 GATC ^GATC
NgoMIV 539 GCCGGC G^CCGGC
NotI 605 GCGGCCGC GC^GGCCGC
PflFI 57 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 859 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 577 CTGCAG CTGCA^G
SacI 401 GAGCTC GAGCT^C
SapI 472 GAAGAGC GCTCTTC(1/4)
Sau3AI 510 GATC ^GATC
SfcI 577 CTGCAG C^T[GA][CT]AG
SmlI 244 CTCAAG C^T[CT][GA]AG
StuI 590 AGGCCT AGG^CCT
Tsp45I 55 GTGAC ^GT[GC]AC
Tth111I 57 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 636 CCAGCCCCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 509 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 196 GGTCC G^G[AT]CC
958 GGACC
BsmFI 77 GGGAC GGGAC(10/14)
957 GGGAC
BsmI 516 GAATGC GAATGC(1/-1)
543 GCATTC
DraII 195 GGGTCCT [GA]G^G[ACGT]CC[CT]
957 GGGACCC
EarI 443 CTCTTC CTCTTC(1/4)
472 GAAGAG
EcoO109I 195 GGGTCCT [GA]G^G[ACGT]CC[CT]
957 GGGACCC
HgaI 670 GACGC GACGC(5/10)
864 GCGTC
Ksp632I 443 CTCTTC CTCTTC(1/4)
472 GAAGAG
MaeII 725 ACGT A^CGT
886 ACGT
MslI 789 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
885 CACGTCCATG
NlaIII 600 CATG CATG^
891 CATG
PpuMI 195 GGGTCCT [GA]G^G[AT]CC[CT]
957 GGGACCC
SmaI 569 CCCGGG CCC^GGG
998 CCCGGG
XmaCI 569 CCCGGG C^CCGGG
998 CCCGGG
XmaI 569 CCCGGG C^CCGGG
998 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
BanI BanII BbvI BcnI BglI
BmyI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIV PvuII
RsaI Sau96I ScrFI SfaNI TseI