Restriction map for
pF1KA0678
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp700I Asp718I
AvaI AviII AvrII BamHI BbrPI
BcgI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BstEII BstPI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HpaI KpnI KspI
MfeI MluI MroI MunI NaeI
NgoMIV NotI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 3623 GGCGCC G[GA]^CG[CT]C
AhdI 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2709 GTGCAC G^TGCAC
ApaLI 2709 GTGCAC G^TGCAC
AspEI 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3623 GGCGCC GGCGC^C
BbvCI 6115 CCTCAGC CCTCAGC(-5/-2)
BcnI 3822 CCCGG CC^[GC]GG
BglI 6460 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4823 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4823 GCTTAGC GC^T[ACGT]AGC
BsaHI 3623 GGCGCC G[GA]^CG[CT]C
BsaWI 3794 TCCGGT [AT]^CCGG[AT]
BsgI 5365 GTGCAG GTGCAG(16/14)
BssSI 2339 CACGAG CACGAG(-5/-1)
BstXI 5450 CCAGTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4823 GCTTAGC GC^T[ACGT]AGC
DraIII 3156 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3101 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 640 GAATTC G^AATTC
HaeII 3623 GGCGCC [GA]GCGC^[CT]
Hin1I 3623 GGCGCC G[GA]^CG[CT]C
KasI 3623 GGCGCC G^GCGCC
NarI 3623 GGCGCC GG^CGCC
NciI 3822 CCCGG CC^[GC]GG
NdeI 4046 CATATG CA^TATG
NheI 4272 GCTAGC G^CTAGC
NruI 1353 TCGCGA TCG^CGA
PflFI 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3020 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3227 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 438 AGTACT AGT^ACT
SfoI 3623 GGCGCC GGC^GCC
Tth111I 5413 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3020 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AflII 1470 CTTAAG C^TTAAG
3076 CTTAAG
AocI 1074 CCTCAGG CC^T[ACGT]AGG
5869 CCTGAGG
AseI 418 ATTAAT AT^TAAT
6337 ATTAAT
AsnI 418 ATTAAT AT^TAAT
6337 ATTAAT
BalI 3808 TGGCCA TGG^CCA
5186 TGGCCA
BfrI 1470 CTTAAG C^TTAAG
3076 CTTAAG
BglII 480 AGATCT A^GATCT
2001 AGATCT
BsaBI 2020 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4000 GATAAGAATC
BsaI 6268 GAGACC GGTCTC(1/5)
1180 GGTCTC
BseRI 1296 GAGGAG GAGGAG(10/8)
5942 GAGGAG
Bsp1407I 3389 TGTACA T^GTACA
5745 TGTACA
BspMI 6680 ACCTGC ACCTGC(4/8)
6652 GCAGGT
BsrGI 3389 TGTACA T^GTACA
5745 TGTACA
Bst1107I 509 GTATAC GTA^TAC
3497 GTATAC
BstAPI 5125 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5554 GCAAAGGGTGC
BstBI 2234 TTCGAA TT^CGAA
5675 TTCGAA
BstZ17I 509 GTATAC GTA^TAC
3497 GTATAC
Bsu36I 1074 CCTCAGG CC^T[ACGT]AGG
5869 CCTGAGG
CfoI 3624 GCGC GCG^C
6107 GCGC
DsaI 1721 CCATGG C^C[GA][CT]GG
6254 CCATGG
EaeI 3808 TGGCCA [CT]^GGCC[GA]
5186 TGGCCA
Eco81I 1074 CCTCAGG CC^T[ACGT]AGG
5869 CCTGAGG
EcoNI 2460 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4611 CCTATTTCAGG
HgaI 547 GCGTC GACGC(5/10)
1359 GCGTC
HhaI 3624 GCGC GCG^C
6107 GCGC
HinP1I 3624 GCGC G^CGC
6107 GCGC
HincII 4288 GTCAAC GT[CT]^[GA]AC
5419 GTCAAC
HindII 4288 GTCAAC GT[CT]^[GA]AC
5419 GTCAAC
MaeII 792 ACGT A^CGT
5683 ACGT
MamI 2020 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4000 GATAAGAATC
MluNI 3808 TGGCCA TGG^CCA
5186 TGGCCA
MscI 3808 TGGCCA TGG^CCA
5186 TGGCCA
MspA1I 1925 CAGCTG C[AC]G^C[GT]G
6118 CAGCTG
NcoI 1721 CCATGG C^CATGG
6254 CCATGG
NspV 2234 TTCGAA TT^CGAA
5675 TTCGAA
PshBI 418 ATTAAT AT^TAAT
6337 ATTAAT
PvuII 1925 CAGCTG CAG^CTG
6118 CAGCTG
SacI 4315 GAGCTC GAGCT^C
6034 GAGCTC
SapI 4312 GAAGAGC GCTCTTC(1/4)
5461 GCTCTTC
SfuI 2234 TTCGAA TT^CGAA
5675 TTCGAA
SphI 1040 GCATGC GCATG^C
4832 GCATGC
SspBI 3389 TGTACA T^GTACA
5745 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BciVI BclI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII