Restriction map for
pF1KB8648
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI CelII CfoI
Cfr10I CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 22 GCGG CCGC(-3/-1)
AlwNI 437 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 507 CCTTAGG CC^T[ACGT]AGG
BbrPI 572 CACGTG CAC^GTG
BcnI 308 CCGGG CC^[GC]GG
BpmI 995 CTGGAG CTGGAG(16/14)
BsaAI 572 CACGTG [CT]AC^GT[GA]
BseRI 920 GAGGAG GAGGAG(10/8)
BspDI 664 ATCGAT AT^CGAT
Bst1107I 149 GTATAC GTA^TAC
BstAPI 985 GCAATAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1008 GGATG GGATG(2/0)
BstYI 846 AGATCC [GA]^GATC[CT]
BstZ17I 149 GTATAC GTA^TAC
Bsu36I 507 CCTTAGG CC^T[ACGT]AGG
Cfr13I 738 GGCCC G^G[ACGT]CC
ClaI 664 ATCGAT AT^CGAT
DsaI 705 CCGTGG C^C[GA][CT]GG
Eco81I 507 CCTTAGG CC^T[ACGT]AGG
EcoT14I 1013 CCAAGG C^C[AT][AT]GG
FokI 1008 GGATG GGATG(9/13)
HapII 308 CCGG C^CGG
HpaII 308 CCGG C^CGG
HphI 302 TCACC GGTGA(8/7)
MflI 846 AGATCC [GA]^GATC[CT]
MspI 308 CCGG C^CGG
NciI 308 CCGGG CC^[GC]GG
NlaIV 311 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 436 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 572 CACGTG CAC^GTG
PmlI 572 CACGTG CAC^GTG
SapI 167 GAAGAGC GCTCTTC(1/4)
Sau96I 738 GGCCC G^G[ACGT]CC
ScaI 549 AGTACT AGT^ACT
SpeI 583 ACTAGT A^CTAGT
StyI 1013 CCAAGG C^C[AT][AT]GG
TspRI 699 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 436 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 630 CCATGAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 846 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 319 GAATTT [GA]^AATT[CT]
379 GAATTT
ApoI 319 GAATTT [GA]^AATT[CT]
379 GAATTT
AspHI 170 GAGCTC G[AT]GC[AT]^C
925 GAGCTC
BanII 170 GAGCTC G[GA]GC[CT]^C
925 GAGCTC
BfaI 334 CTAG C^TAG
584 CTAG
BmyI 170 GAGCTC G[AGT]GC[ACT]^C
925 GAGCTC
BsiHKAI 170 GAGCTC G[AT]GC[AT]^C
925 GAGCTC
BsiYI 436 CCAGGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCCATTGGGGG
BslI 436 CCAGGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
859 CCCATTGGGGG
BsmFI 512 GGGAC GGGAC(10/14)
867 GGGAC
Bsp1286I 170 GAGCTC G[AGT]GC[ACT]^C
925 GAGCTC
BsrDI 516 CATTGC GCAATG(2/0)
179 GCAATG
BsrI 388 ACTGG ACTGG(1/-1)
552 ACTGG
Cac8I 640 GCTGGC GC[ACGT]^[ACGT]GC
905 GCTTGC
EaeI 702 TGGCCG [CT]^GGCC[GA]
716 TGGCCG
HindIII 368 AAGCTT A^AGCTT
903 AAGCTT
MaeI 334 CTAG C^TAG
584 CTAG
MwoI 976 GCCACCTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
985 GCAATAACTGC
PleI 191 GACTC GAGTC(4/5)
755 GACTC
SacI 170 GAGCTC GAGCT^C
925 GAGCTC
TaqI 665 TCGA T^CGA
687 TCGA
TthHB8I 665 TCGA T^CGA
687 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccI AfaI AluI AlwI BbvI
BsaJI BssKI BstNI DdeI DpnII
EarI EcoRII Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I