Restriction map for
pF1KB7584
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AhdI 319 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 130 AGCGCT AGC^GCT
ApaI 832 GGGCCC GGGCC^C
AspEI 319 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 586 GAGCAC G[AT]GC[AT]^C
AviII 356 TGCGCA TGC^GCA
BalI 168 TGGCCA TGG^CCA
BanI 576 GGTGCC G^G[CT][GA]CC
BbsI 102 GTCTTC GAAGAC(2/6)
BbvCI 799 CCTCAGC CCTCAGC(-5/-2)
BlpI 24 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTCAGC GC^T[ACGT]AGC
BpuAI 102 GTCTTC GAAGAC(2/6)
BseRI 337 GAGGAG GAGGAG(10/8)
BsgI 814 CTGCAC GTGCAG(16/14)
BsiEI 15 CGATCG CG[GA][CT]^CG
BsiHKAI 586 GAGCAC G[AT]GC[AT]^C
BsrBI 700 CCGCTC CCGCTC(-3/-3)
BsrFI 275 ACCGGC [GA]^CCGG[CT]
BssHII 474 GCGCGC G^CGCGC
BstAPI 300 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 33 CATCC GGATG(2/0)
CelII 24 GCTCAGC GC^T[ACGT]AGC
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
DpnII 16 GATC ^GATC
DsaI 611 CCATGG C^C[GA][CT]GG
Eam1105I 319 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 654 CTCTTC CTCTTC(1/4)
Eco47III 130 AGCGCT AGC^GCT
FokI 33 CATCC GGATG(9/13)
FspI 356 TGCGCA TGC^GCA
Ksp632I 654 CTCTTC CTCTTC(1/4)
MaeIII 109 GTCAC ^GT[ACGT]AC
MboI 16 GATC ^GATC
MluNI 168 TGGCCA TGG^CCA
MscI 168 TGGCCA TGG^CCA
NcoI 611 CCATGG C^CATGG
NdeII 16 GATC ^GATC
PpuMI 317 AGGACCC [GA]G^G[AT]CC[CT]
PstI 430 CTGCAG CTGCA^G
PvuI 15 CGATCG CGAT^CG
Sau3AI 16 GATC ^GATC
SfaNI 32 GCATC GCATC(5/9)
SmaI 829 CCCGGG CCC^GGG
TaqI 14 TCGA T^CGA
TfiI 607 GATTC G^A[AT]TC
Tsp45I 109 GTCAC ^GT[GC]AC
Tsp509I 203 AATT ^AATT
TthHB8I 14 TCGA T^CGA
XcmI 410 CCAGCTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 829 CCCGGG C^CCGGG
XmaI 829 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 72 GGGCTC G[GA]GC[CT]^C
832 GGGCCC
BcnI 829 CCCGG CC^[GC]GG
830 CCGGG
BglI 65 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
478 GCCGAGCTGGC
BsmFI 647 GGGAC GGGAC(10/14)
593 GTCCC
BsrI 322 CCAGT ACTGG(1/-1)
453 CCAGT
BstXI 159 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
722 CCAGCTACCTGG
DraII 317 AGGACCC [GA]G^G[ACGT]CC[CT]
832 GGGCCCC
EcoO109I 317 AGGACCC [GA]G^G[ACGT]CC[CT]
832 GGGCCCC
EcoT14I 77 CCAAGG C^C[AT][AT]GG
611 CCATGG
MboII 103 TCTTC GAAGA(8/7)
655 TCTTC
NciI 829 CCCGG CC^[GC]GG
830 CCGGG
PflMI 453 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
743 CCAACTCCTGG
PleI 82 GACTC GAGTC(4/5)
100 GAGTC
SfcI 126 CTACAG C^T[GA][CT]AG
430 CTGCAG
StyI 77 CCAAGG C^C[AT][AT]GG
611 CCATGG
Van91I 453 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
743 CCAACTCCTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AvaII BbvI BmyI BpmI
BsaJI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI TseI TspRI