Restriction map for
pF1KE0503
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanII BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BsaI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspHI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MseI MslI MunI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 12 CGCG CG^CG
AccIII 548 TCCGGA T^CCGGA
AcyI 16 GGCGCC G[GA]^CG[CT]C
AfaI 234 GTAC GT^AC
BamHI 386 GGATCC G^GATCC
BbeI 16 GGCGCC GGCGC^C
BcgI 173 GCACCGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 44 CCCGG CC^[GC]GG
BglI 307 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 493 TACGTG [CT]AC^GT[GA]
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BseAI 548 TCCGGA T^CCGGA
BsgI 190 GTGCAG GTGCAG(16/14)
Bsp1407I 233 TGTACA T^GTACA
BspEI 548 TCCGGA T^CCGGA
BsrFI 320 GCCGGC [GA]^CCGG[CT]
BsrGI 233 TGTACA T^GTACA
BstBI 181 TTCGAA TT^CGAA
BstF5I 546 CATCC GGATG(2/0)
BstUI 12 CGCG CG^CG
Cfr10I 320 GCCGGC [GA]^CCGG[CT]
DraII 603 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 90 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 603 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 20 CCTTGG C^C[AT][AT]GG
FokI 546 CATCC GGATG(9/13)
Hin1I 16 GGCGCC G[GA]^CG[CT]C
KasI 16 GGCGCC G^GCGCC
MaeII 494 ACGT A^CGT
MboII 417 GAAGA GAAGA(8/7)
MroI 548 TCCGGA T^CCGGA
MvnI 12 CGCG CG^CG
NaeI 320 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NciI 44 CCCGG CC^[GC]GG
NgoMIV 320 GCCGGC G^CCGGC
NspV 181 TTCGAA TT^CGAA
PpuMI 603 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 67 CAGCTG CAG^CTG
RsaI 234 GTAC GT^AC
SfoI 16 GGCGCC GGC^GCC
SfuI 181 TTCGAA TT^CGAA
SspBI 233 TGTACA T^GTACA
StuI 314 AGGCCT AGG^CCT
StyI 20 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Aor51HI 5 AGCGCT AGC^GCT
122 AGCGCT
BfaI 367 CTAG C^TAG
598 CTAG
BlpI 141 GCTGAGC GC^T[ACGT]AGC
352 GCTCAGC
Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC
352 GCTCAGC
BseRI 25 GAGGAG GAGGAG(10/8)
394 GAGGAG
BsmFI 605 GTCCC GGGAC(10/14)
652 GTCCC
BspMI 332 ACCTGC ACCTGC(4/8)
533 ACCTGC
BstAPI 264 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
279 GCAGGCGCTGC
BstYI 206 AGATCC [GA]^GATC[CT]
386 GGATCC
CelII 141 GCTGAGC GC^T[ACGT]AGC
352 GCTCAGC
Eco47III 5 AGCGCT AGC^GCT
122 AGCGCT
HphI 93 GGTGA GGTGA(8/7)
218 TCACC
MaeI 367 CTAG C^TAG
598 CTAG
MflI 206 AGATCC [GA]^GATC[CT]
386 GGATCC
MspA1I 67 CAGCTG C[AC]G^C[GT]G
400 CAGCGG
PstI 523 CTGCAG CTGCA^G
556 CTGCAG
SfaNI 519 GATGC GCATC(5/9)
264 GCATC
Tsp45I 496 GTGAC ^GT[GC]AC
632 GTGAC
TspRI 464 CACTG CA[GC]TG[ACGT][ACGT]^
628 CAGTG
XhoII 206 AGATCC [GA]^GATC[CT]
386 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanI BbvI BmyI BpmI
BsaJI BsaWI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboI MnlI MspI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfcI TaqI TseI TthHB8I