Restriction map for
pF1KE0524
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI MamI MboI MfeI
MflI MluI MseI MunI NarI
NciI NcoI NdeI NdeII NheI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI Sau3AI ScaI
SexAI SfaNI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 559 GACGTC GACGT^C
AccIII 146 TCCGGA T^CCGGA
AfaI 387 GTAC GT^AC
AflIII 389 ACGTGT A^C[GA][CT]GT
ApaI 193 GGGCCC GGGCC^C
AspI 286 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 410 CTCGGG C^[CT]CG[GA]G
AvaII 513 GGACC G^G[AT]CC
BalI 374 TGGCCA TGG^CCA
BbvCI 105 CCTCAGC CCTCAGC(-5/-2)
BfaI 16 CTAG C^TAG
BsaAI 388 TACGTG [CT]AC^GT[GA]
BsaWI 146 TCCGGA [AT]^CCGG[AT]
BseAI 146 TCCGGA T^CCGGA
BseRI 103 CTCCTC GAGGAG(10/8)
BsgI 91 GTGCAG GTGCAG(16/14)
BsoBI 410 CTCGGG C^[CT]CG[GA]G
BspEI 146 TCCGGA T^CCGGA
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BssSI 508 CTCGTG CACGAG(-5/-1)
BstF5I 504 CATCC GGATG(2/0)
BstXI 503 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 469 GCGC GCG^C
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DdeI 106 CTCAG C^T[ACGT]AG
DraIII 136 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 357 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 463 CCTTGG C^C[AT][AT]GG
FokI 504 CATCC GGATG(9/13)
HaeII 468 GGCGCT [GA]GCGC^[CT]
HhaI 469 GCGC GCG^C
HinP1I 469 GCGC G^CGC
HincII 421 GTCAAC GT[CT]^[GA]AC
HindII 421 GTCAAC GT[CT]^[GA]AC
MaeI 16 CTAG C^TAG
MluNI 374 TGGCCA TGG^CCA
MroI 146 TCCGGA T^CCGGA
MscI 374 TGGCCA TGG^CCA
NaeI 39 GCCGGC GCC^GGC
NgoMIV 39 GCCGGC G^CCGGC
NotI 272 GCGGCCGC GC^GGCCGC
PflFI 286 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 335 GACTC GAGTC(4/5)
RsaI 387 GTAC GT^AC
SapI 225 GCTCTTC GCTCTTC(1/4)
SbfI 30 CCTGCAGG CCTGCA^GG
SfiI 366 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 248 GCATGC GCATG^C
Sse8387I 30 CCTGCAGG CCTGCA^GG
StyI 463 CCTTGG C^C[AT][AT]GG
TfiI 203 GATTC G^A[AT]TC
Tth111I 286 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 313 GGCGTC G[GA]^CG[CT]C
559 GACGTC
AspHI 100 GAGCTC G[AT]GC[AT]^C
700 GAGCTC
BbsI 292 GTCTTC GAAGAC(2/6)
562 GTCTTC
BglI 367 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
674 GCCAGGGCGGC
BpuAI 292 GTCTTC GAAGAC(2/6)
562 GTCTTC
BsaHI 313 GGCGTC G[GA]^CG[CT]C
559 GACGTC
BsiHKAI 100 GAGCTC G[AT]GC[AT]^C
700 GAGCTC
BspMI 515 ACCTGC ACCTGC(4/8)
33 GCAGGT
DsaI 86 CCGCGG C^C[GA][CT]GG
270 CCGCGG
FauI 132 CCCGC CCCGC(4/6)
51 GCGGG
HapII 40 CCGG C^CGG
147 CCGG
HgaI 48 GACGC GACGC(5/10)
314 GCGTC
Hin1I 313 GGCGTC G[GA]^CG[CT]C
559 GACGTC
HinfI 203 GATTC G^A[ACGT]TC
335 GACTC
HpaII 40 CCGG C^CGG
147 CCGG
KspI 86 CCGCGG CCGC^GG
270 CCGCGG
MaeII 389 ACGT A^CGT
560 ACGT
MaeIII 46 GTGAC ^GT[ACGT]AC
172 GTCAC
MspI 40 CCGG C^CGG
147 CCGG
NspI 221 ACATGC [GA]CATG^[CT]
248 GCATGC
PstI 31 CTGCAG CTGCA^G
595 CTGCAG
PvuII 94 CAGCTG CAG^CTG
638 CAGCTG
SacI 100 GAGCTC GAGCT^C
700 GAGCTC
SacII 86 CCGCGG CCGC^GG
270 CCGCGG
SfcI 31 CTGCAG C^T[GA][CT]AG
595 CTGCAG
Tsp45I 46 GTGAC ^GT[GC]AC
172 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI BanI BanII
BbvI BmyI BpmI BsaJI BsiEI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I Cfr13I DraII
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI Ksp632I MboII MnlI MslI
MspA1I MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI TseI