Restriction map for
pF1KB8664
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI DraI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsaI
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 158 GACGTC GACGT^C
AccII 1008 CGCG CG^CG
AcyI 158 GACGTC G[GA]^CG[CT]C
ApaI 1182 GGGCCC GGGCC^C
Asp700I 984 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 904 GTGCTC G[AT]GC[AT]^C
AvaI 964 CCCGGG C^[CT]CG[GA]G
AvaII 1157 GGACC G^G[AT]CC
BanI 364 GGCACC G^G[CT][GA]CC
BpmI 79 CTGGAG CTGGAG(16/14)
BsaBI 807 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 158 GACGTC G[GA]^CG[CT]C
BsaI 933 GAGACC GGTCTC(1/5)
BsaWI 1154 ACCGGA [AT]^CCGG[AT]
BsgI 130 CTGCAC GTGCAG(16/14)
BsiHKAI 904 GTGCTC G[AT]GC[AT]^C
BsoBI 964 CCCGGG C^[CT]CG[GA]G
BspHI 723 TCATGA T^CATGA
BsrFI 572 ACCGGC [GA]^CCGG[CT]
BssSI 543 CACGAG CACGAG(-5/-1)
BstEII 102 GGTCACC G^GT[ACGT]ACC
BstPI 102 GGTCACC G^GT[ACGT]ACC
BstUI 1008 CGCG CG^CG
Cfr10I 572 ACCGGC [GA]^CCGG[CT]
CpoI 1156 CGGACCG CG^G[AT]CCG
DraIII 295 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1186 CCACGG C^C[GA][CT]GG
EcoO65I 102 GGTCACC G^GT[ACGT]ACC
HgaI 1006 GACGC GACGC(5/10)
Hin1I 158 GACGTC G[GA]^CG[CT]C
HincII 1123 GTCAAC GT[CT]^[GA]AC
HindII 1123 GTCAAC GT[CT]^[GA]AC
HinfI 1173 GATTC G^A[ACGT]TC
MaeII 159 ACGT A^CGT
MamI 807 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1008 CGCG CG^CG
PshAI 1191 GACGATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 846 CAGCTG CAG^CTG
RcaI 723 TCATGA T^CATGA
RsrII 1156 CGGACCG CG^G[AT]CCG
SmaI 964 CCCGGG CCC^GGG
SmlI 169 CTCAAG C^T[CT][GA]AG
StuI 392 AGGCCT AGG^CCT
TfiI 1173 GATTC G^A[AT]TC
XmaCI 964 CCCGGG C^CCGGG
XmaI 964 CCCGGG C^CCGGG
XmnI 984 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1063 GTAGAC GT^[AC][GT]AC
1147 GTAGAC
AcsI 319 GAATTC [GA]^AATT[CT]
505 GAATTC
ApoI 319 GAATTC [GA]^AATT[CT]
505 GAATTC
BbsI 674 GAAGAC GAAGAC(2/6)
984 GAAGAC
BbvCI 885 CCTCAGC CCTCAGC(-5/-2)
91 GCTGAGG
BpuAI 674 GAAGAC GAAGAC(2/6)
984 GAAGAC
BsmBI 1116 CGTCTC CGTCTC(1/5)
247 GAGACG
BsmFI 702 GTCCC GGGAC(10/14)
731 GTCCC
Cac8I 28 GCTGGC GC[ACGT]^[ACGT]GC
611 GCAAGC
DpnII 807 GATC ^GATC
813 GATC
EarI 337 CTCTTC CTCTTC(1/4)
930 GAAGAG
EcoRI 319 GAATTC G^AATTC
505 GAATTC
FauI 50 GCGGG CCCGC(4/6)
59 GCGGG
Ksp632I 337 CTCTTC CTCTTC(1/4)
930 GAAGAG
MboI 807 GATC ^GATC
813 GATC
MseI 149 TTAA T^TAA
257 TTAA
MslI 298 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
489 CACCTTCATG
NdeII 807 GATC ^GATC
813 GATC
NspI 600 GCATGT [GA]CATG^[CT]
1143 GCATGT
Sau3AI 807 GATC ^GATC
813 GATC
SfaNI 499 GCATC GCATC(5/9)
590 GCATC
Tru9I 149 TTAA T^TAA
257 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PstI
Sau96I ScrFI SfcI TseI Tsp45I
Tsp509I TspRI