Restriction map for
pF1KB6509
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 130 CGCG CG^CG
AlwNI 457 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 561 GAGCTC G[AT]GC[AT]^C
AvrII 147 CCTAGG C^CTAGG
BalI 794 TGGCCA TGG^CCA
BanI 544 GGCACC G^G[CT][GA]CC
BbsI 653 GTCTTC GAAGAC(2/6)
BcnI 996 CCCGG CC^[GC]GG
BlnI 147 CCTAGG C^CTAGG
BpmI 365 CTGGAG CTGGAG(16/14)
BpuAI 653 GTCTTC GAAGAC(2/6)
BsaAI 1042 TACGTG [CT]AC^GT[GA]
BsiHKAI 561 GAGCTC G[AT]GC[AT]^C
BsmAI 296 GAGAC GTCTC(1/5)
BsmI 641 GCATTC GAATGC(1/-1)
BspMI 260 ACCTGC ACCTGC(4/8)
BsrDI 618 CATTGC GCAATG(2/0)
BstUI 130 CGCG CG^CG
BstYI 231 GGATCT [GA]^GATC[CT]
CfoI 1047 GCGC GCG^C
EaeI 794 TGGCCA [CT]^GGCC[GA]
EcoT14I 147 CCTAGG C^C[AT][AT]GG
HapII 997 CCGG C^CGG
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HindIII 838 AAGCTT A^AGCTT
HpaII 997 CCGG C^CGG
MaeII 1043 ACGT A^CGT
MflI 231 GGATCT [GA]^GATC[CT]
MluNI 794 TGGCCA TGG^CCA
MscI 794 TGGCCA TGG^CCA
MseI 302 TTAA T^TAA
MspI 997 CCGG C^CGG
MvnI 130 CGCG CG^CG
NciI 996 CCCGG CC^[GC]GG
NheI 715 GCTAGC G^CTAGC
NlaIII 472 CATG CATG^
NlaIV 544 GGCACC GG[ACGT]^[ACGT]CC
PleI 981 GAGTC GAGTC(4/5)
PvuII 418 CAGCTG CAG^CTG
SacI 561 GAGCTC GAGCT^C
SapI 558 GAAGAGC GCTCTTC(1/4)
ScaI 1004 AGTACT AGT^ACT
SexAI 852 ACCTGGT A^CC[AT]GGT
SfcI 914 CTGTAG C^T[GA][CT]AG
SmlI 647 CTCAAG C^T[CT][GA]AG
StyI 147 CCTAGG C^C[AT][AT]GG
Tru9I 302 TTAA T^TAA
Tsp45I 929 GTGAC ^GT[GC]AC
XhoII 231 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 889 GTCTAC GT^[AC][GT]AC
1039 GTCTAC
AlwI 231 GGATC GGATC(4/5)
922 GGATC
BanII 139 GAGCCC G[GA]GC[CT]^C
561 GAGCTC
BbvI 712 GCAGC GCAGC(8/12)
462 GCTGC
BsiYI 142 CCCTCCCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1002 CCAGTACTGGG
BslI 142 CCCTCCCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1002 CCAGTACTGGG
EarI 452 CTCTTC CTCTTC(1/4)
558 GAAGAG
Fnu4HI 462 GCTGC GC^[ACGT]GC
712 GCAGC
HincII 337 GTCAAC GT[CT]^[GA]AC
778 GTTGAC
HindII 337 GTCAAC GT[CT]^[GA]AC
778 GTTGAC
ItaI 462 GCTGC GC^[ACGT]GC
712 GCAGC
Ksp632I 452 CTCTTC CTCTTC(1/4)
558 GAAGAG
MaeIII 502 GTTAC ^GT[ACGT]AC
929 GTGAC
SfaNI 709 GATGC GCATC(5/9)
767 GCATC
TaqI 137 TCGA T^CGA
186 TCGA
TfiI 330 GATTC G^A[AT]TC
414 GAATC
TseI 462 GCTGC G^C[AT]GC
712 GCAGC
TspRI 155 CAGTG CA[GC]TG[ACGT][ACGT]^
196 CAGTG
TthHB8I 137 TCGA T^CGA
186 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI AvaII BfaI
BmyI BsaJI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII FokI HaeIII HinfI
HphI MaeI MboI MboII MnlI
MslI MspA1I MvaI MwoI NdeII
RsaI Sau3AI Sau96I ScrFI Tsp509I